Mostrar el registro sencillo del ítem

dc.contributor.author Ibañez, Maria Silvina
dc.contributor.author Camadro, Elsa Lucila
dc.contributor.author Sala, Carlos A.
dc.contributor.author Masuelli, Ricardo Williams
dc.date.available 2017-08-25T19:31:57Z
dc.date.issued 2014-01
dc.identifier.citation Ibañez, Maria Silvina; Camadro, Elsa Lucila; Sala, Carlos A.; Masuelli, Ricardo Williams; Morphological and molecular diversity of the wild carrot Daucus pusillus: implications for classification and ex situ conservation; Natl Research Council Canada-n R C Research Press; Botany; 92; 5; 1-2014; 348-359
dc.identifier.issn 1916-2790
dc.identifier.uri http://hdl.handle.net/11336/23017
dc.description.abstract Daucus pusillus Michx. and Daucus montevidensis Link ex Spreng. are wild carrots from the Americas with unresolved taxonomic status. An investigation was carried out with accessions of D. pusillus and (or) D. montevidensis from Argentina for (i) morphological and molecular (amplified fragments length polymorphism (AFLP) and inter simple sequence repeat (ISSR)) characterization, (ii) analysis of congruence of morphological and molecular variation, and (iii) comparison of diversity for the internal transcribed spacer (ITS) region with that reported for a North American accession of D. pusillus. Twelve accessions of D. pusillus and (or) D. montevidensis ? representing their geographical distribution in Argentina ? and one accession each of wild Daucus carota L. and Daucus montanus Humb. & Bonpl. ex Schult. ? as outgroups ? were included. In the multivariate analysis of morphological diversity, two accessions were clearly differentiated; this result is not sustained by multivariate analysis of molecular diversity. Based on multivariate and molecular variance (AMOVA) analyses, D. pusillus and (or) D. montevidensis accessions were separated at the molecular level into two groups, associated with geographical origin. Because this result is not supported by morphology, the segregation into two taxa seems unjustified. In all accessions, ITS and 5.8S rDNA regions had identical sequences, which differ in one nucleotide from the corresponding sequence of the North American accession. According to the combined results, D. pusillus would be a single taxon distributed from North to South America, and D. montevidensis a nomenclatural synonym. Autogamy of D. pusillus and its highly structured genetic diversity (Fst = 0.86) allows the application of a geographically targeted approach for germplasm exploration, conservation and eventual use in pre-breeding.
dc.format application/pdf
dc.language.iso eng
dc.publisher Natl Research Council Canada-n R C Research Press
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject wild carrots
dc.subject genetics resources
dc.subject Molecular diversity
dc.subject morphological diversity
dc.subject ITS sequence
dc.subject.classification Bioquímica y Biología Molecular
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Morphological and molecular diversity of the wild carrot Daucus pusillus: implications for classification and ex situ conservation
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-07-13T18:15:25Z
dc.journal.volume 92
dc.journal.number 5
dc.journal.pagination 348-359
dc.journal.pais Canadá
dc.journal.ciudad Otawa
dc.description.fil Fil: Ibañez, Maria Silvina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Camadro, Elsa Lucila. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Sala, Carlos A.. Nidera S.A. Departamento de Biotecnología; Argentina
dc.description.fil Fil: Masuelli, Ricardo Williams. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina
dc.journal.title Botany
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1139/cjb-2013-0241
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.nrcresearchpress.com/doi/abs/10.1139/cjb-2013-0241#.WaB7Bvk1-JA


Archivos asociados

Icon
Blocked Acceso no disponible

Aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem

info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)