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dc.contributor.author
Ibañez, Maria Silvina
dc.contributor.author
Camadro, Elsa Lucila
dc.contributor.author
Sala, Carlos A.
dc.contributor.author
Masuelli, Ricardo Williams
dc.date.available
2017-08-25T19:31:57Z
dc.date.issued
2014-01
dc.identifier.citation
Ibañez, Maria Silvina; Camadro, Elsa Lucila; Sala, Carlos A.; Masuelli, Ricardo Williams; Morphological and molecular diversity of the wild carrot Daucus pusillus: implications for classification and ex situ conservation; Natl Research Council Canada-n R C Research Press; Botany; 92; 5; 1-2014; 348-359
dc.identifier.issn
1916-2790
dc.identifier.uri
http://hdl.handle.net/11336/23017
dc.description.abstract
Daucus pusillus Michx. and Daucus montevidensis Link ex Spreng. are wild carrots from the Americas with unresolved taxonomic status. An investigation was carried out with accessions of D. pusillus and (or) D. montevidensis from Argentina for (i) morphological and molecular (amplified fragments length polymorphism (AFLP) and inter simple sequence repeat (ISSR)) characterization, (ii) analysis of congruence of morphological and molecular variation, and (iii) comparison of diversity for the internal transcribed spacer (ITS) region with that reported for a North American accession of D. pusillus. Twelve accessions of D. pusillus and (or) D. montevidensis ? representing their geographical distribution in Argentina ? and one accession each of wild Daucus carota L. and Daucus montanus Humb. & Bonpl. ex Schult. ? as outgroups ? were included. In the multivariate analysis of morphological diversity, two accessions were clearly differentiated; this result is not sustained by multivariate analysis of molecular diversity. Based on multivariate and molecular variance (AMOVA) analyses, D. pusillus and (or) D. montevidensis accessions were separated at the molecular level into two groups, associated with geographical origin. Because this result is not supported by morphology, the segregation into two taxa seems unjustified. In all accessions, ITS and 5.8S rDNA regions had identical sequences, which differ in one nucleotide from the corresponding sequence of the North American accession. According to the combined results, D. pusillus would be a single taxon distributed from North to South America, and D. montevidensis a nomenclatural synonym. Autogamy of D. pusillus and its highly structured genetic diversity (Fst = 0.86) allows the application of a geographically targeted approach for germplasm exploration, conservation and eventual use in pre-breeding.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Natl Research Council Canada-n R C Research Press
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Wild Carrots
dc.subject
Genetics Resources
dc.subject
Molecular Diversity
dc.subject
Morphological Diversity
dc.subject
Its Sequence
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Morphological and molecular diversity of the wild carrot Daucus pusillus: implications for classification and ex situ conservation
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-07-13T18:15:25Z
dc.journal.volume
92
dc.journal.number
5
dc.journal.pagination
348-359
dc.journal.pais
Canadá
dc.journal.ciudad
Otawa
dc.description.fil
Fil: Ibañez, Maria Silvina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Camadro, Elsa Lucila. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Sala, Carlos A.. Nidera S.A. Departamento de Biotecnología; Argentina
dc.description.fil
Fil: Masuelli, Ricardo Williams. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina
dc.journal.title
Botany
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1139/cjb-2013-0241
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.nrcresearchpress.com/doi/abs/10.1139/cjb-2013-0241#.WaB7Bvk1-JA


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