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dc.contributor.author
Liang, Wangzhuang
dc.contributor.author
Dong, Huixue
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Guo, Xiaojiang
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Rodríguez, María Verónica
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Cheng, Mengping
dc.contributor.author
Li, Maolian
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Benech-Arnold, Roberto Luis
dc.contributor.author
Pu, Zhien
dc.contributor.author
Wang, Jirui
dc.date.available
2024-02-28T13:04:38Z
dc.date.issued
2023-07
dc.identifier.citation
Liang, Wangzhuang; Dong, Huixue; Guo, Xiaojiang; Rodríguez, María Verónica; Cheng, Mengping; et al.; Identification of long-lived and stable mRNAs in the aged seeds of wheat; Maximum Academic Press; Seed Biology; 2; 14; 7-2023; 1-13
dc.identifier.issn
2834-5495
dc.identifier.uri
http://hdl.handle.net/11336/228777
dc.description.abstract
Seed germination relies on preserving mRNA integrity in dry seeds. However, the quality of mRNA in aged wheat seeds is not well understood. Here, we investigated 20 wheat varieties for seed longevity using controlled deterioration treatment (CDT) and identified that Chinese Spring seeds exhibit moderate longevity. We observed correlations between seed viability and RNA integrity in the aleurone and embryo cells after aging-treatment. Nanopore sequencing of whole seeds from natural aging treatment (NAT) and CDT for 25 days identified 3,083 full-length transcripts. We performed RNA-seq transcriptome profiling to classify the tissue origin of these transcripts under eight aging treatments, revealing the presence of 2,064 overlapping long-lived mRNAs (LLRs) in the seed embryo and 2,130 in the aleurone layers. These LLRs corresponded to genes with detectable transcription levels and at least one full-length transcript in their coding sequence. Notably, degradation percentages of these mRNAs varied among seeds of different wheat varieties with similar ages. We predicted 21 most stable LLRs with high GC% content and short coding sequence length, among which only one LLR was seed-specifically expressed and belonged to the late-embryogenesis-abundant (LEA) protein family. RT-PCR confirmed the expression of the seven LLR fragments in the aleurone layer and embryo of Chinese Spring seeds. We found three of the most stable LLRs (LLR13, LLR15, and LLR20) identified in Chinese Spring were more stable in high longevity varieties than in short longevity varieties after aging, indicating their potential roles in seed longevity and germination.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Maximum Academic Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
STORED mRNA
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WHEAT
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AGED SEEDS
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LONG LIVED RNA
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EMBRYOS
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ALEURONE LAYER
dc.subject.classification
Ciencias de las Plantas, Botánica
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Identification of long-lived and stable mRNAs in the aged seeds of wheat
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-02-28T10:11:07Z
dc.journal.volume
2
dc.journal.number
14
dc.journal.pagination
1-13
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Fayetteville
dc.description.fil
Fil: Liang, Wangzhuang. Sichuan Agricultural University; China
dc.description.fil
Fil: Dong, Huixue. Sichuan Agricultural University; China
dc.description.fil
Fil: Guo, Xiaojiang. Sichuan Agricultural University; China
dc.description.fil
Fil: Rodríguez, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal; Argentina
dc.description.fil
Fil: Cheng, Mengping. Sichuan Agricultural University; China
dc.description.fil
Fil: Li, Maolian. Sichuan Agricultural University; China
dc.description.fil
Fil: Benech-Arnold, Roberto Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal; Argentina
dc.description.fil
Fil: Pu, Zhien. Sichuan Agricultural University; China
dc.description.fil
Fil: Wang, Jirui. Sichuan Agricultural University; China
dc.journal.title
Seed Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.maxapress.com/article/doi/10.48130/SeedBio-2023-0014
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.48130/SeedBio-2023-0014
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