Evento
GABA and ACh are co-released from olivocochlear efferent terminals
Castagnola, Tais
; Elgoyhen, Ana Belen
; Gomez Casati, Maria Eugenia
; Castagna, Valeria Carolina
; Goutman, Juan Diego
; Wedemeyer, Carolina
Tipo del evento:
Reunión
Nombre del evento:
XXXVII Annual Meeting of the Argentine Society for Research in Neuroscience
Fecha del evento:
18/10/2021
Institución Organizadora:
Sociedad Argentina de Investigación en Neurociencias;
Título del Libro:
XXXVII Annual Meeting of the Argentine Society for Research in Neuroscience
Editorial:
Sociedad Argentina de Investigación en Neurociencias
Idioma:
Inglés
Clasificación temática:
Resumen
During development, inner hair cells (IHCs) in the mammalian cochlea are unresponsive to acoustic stimuli but instead present intrinsic electrical activity, crucial for the normal development of the auditory pathway. During this same period, neurons originating from the medial olivocochlear complex (MOC) transiently innervate IHCs. This innervation is mediated by acetylcholine (ACh), activating nicotinic receptors assembled by α9 and α10 subunits and is responsible for controlling IHC excitability during this period. Even though this is a cholinergic synapse, previous evidence indicates the presence of abundant GABA and GABAB receptors in MOC fibers in the inner spiral bundle. Moreover, the application of GABAB agonists reduces ACh release. Transgenic mice expressing channelrodhopsin (ChR2) under the control of either GAD or ChAT promoters were used in this study. Here we show for the first time, that optogenetically activated fibers in GAD-cre/ChR2 mice (n=7) produced postsynaptic responses that were blocked with cholinergic antagonists (n=3). In addition, pharmacological experiments indicate GABAB activation in ChAT-cre/ChR2 mice (n=4). ChAT-cre/TdTomato cochleas, co-stained with antibody against GAD, showed a co-localization of GABAergic and cholinergic terminals in the inner spiral bundle. Altogether these results strongly suggest that ACh is being co-released with GABA from MOC fibers.
Palabras clave:
COCHLEA
,
EFFERENTS
,
OLIVOCOCHLEAR
,
OPTOGENETIC
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Colecciones
Eventos(INGEBI)
Eventos de INST.DE INVEST.EN ING.GENETICA Y BIOL.MOLECULAR "DR. HECTOR N TORRES"
Eventos de INST.DE INVEST.EN ING.GENETICA Y BIOL.MOLECULAR "DR. HECTOR N TORRES"
Citación
GABA and ACh are co-released from olivocochlear efferent terminals; XXXVII Annual Meeting of the Argentine Society for Research in Neuroscience; Ciudad Autónoma de Buenos Aires; Argentina; 2021; 231-231
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