Artículo
Comparison of MSD analysis from single particle tracking with MSD from images: Getting the best of both worlds
Fecha de publicación:
09/2023
Editorial:
IOP Publishing
Revista:
Methods and Applications in Fluorescence
ISSN:
2050-6120
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Fluorescence microscopy can provide valuable information about cell interior dynamics. Particularly, mean squared displacement (MSD) analysis is widely used to characterize proteins and sub-cellular structures’ mobility providing the laws of molecular diffusion. The MSD curve is traditionally extracted from individual trajectories recorded by single-particle tracking-based techniques. More recently, image correlation methods like iMSD have been shown capable of providing averaged dynamic information directly from images, without the need for isolation and localization of individual particles. iMSD is a powerful technique that has been successfully applied to many different biological problems, over a wide spatial and temporal scales. The aim of this work is to review and compare these two well-established methodologies and their performance in different situations, to give an insight on how to make the most out of their unique characteristics. We show the analysis of the same datasets by the two methods. Regardless of the experimental differences in the input data for MSD or iMSD analysis, our results show that the two approaches can address equivalent questions for free diffusing systems. We focused on studying a range of diffusion coefficients between D = 0.001 μm2 s−1 and D = 0.1 μm2 s−1, where we verified that the equivalence is maintained even for the case of isolated particles. This opens new opportunities for studying intracellular dynamics using equipment commonly available in any biophysical laboratory.
Palabras clave:
FLUORESCENCE
,
MEAN SQUARED DISPLACEMENT
,
OPTICAL MICROSCOPY
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Identificadores
Colecciones
Articulos(IFIBA)
Articulos de INST.DE FISICA DE BUENOS AIRES
Articulos de INST.DE FISICA DE BUENOS AIRES
Citación
Kettmayer, Constanza; Gratton, Enrico; Estrada, Laura Cecilia; Comparison of MSD analysis from single particle tracking with MSD from images: Getting the best of both worlds; IOP Publishing; Methods and Applications in Fluorescence; 12; 1; 9-2023; 1-14
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