Artículo
Azospirillum argentinense Modifies Arabidopsis Root Architecture Through Auxin-dependent Pathway and Flagellin
Mora, Maria Veronica
; Lopez, Gaston Alberto
; Molina, Romina Micaela
; Coniglio, Nayla Anahí
; Nievas, Sofia Mariela
; De Diego, Nuria; Zeljković, Sanja Ćavar; Sarmiento, Sara Salcedo; Spíchal, Luká; Robertson, Sean; Wilkins, Olivia; Elías, Juliana María
; Pedraza, Raúl Osvaldo; Estevez, Jose Manuel
; Belmonte, Mark F.; Cassan, Fabricio Dario
Fecha de publicación:
06/2023
Editorial:
Springer
Revista:
Journal of Soil Science and Plant Nutrition
ISSN:
0718-9508
e-ISSN:
0718-9516
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
To evaluate if root architecture changes observed in Arabidopsis thaliana inoculated with Azospirillum argentinense Az39 depend exclusively on the bacterial capacity to produce indole-3-acetic acid (IAA) and plant ability to sense IAA levels. Azospirillum argentinense Az39, A. argentinense Az39 ipdC–, flagellin from A. argentinense Az39, and pure IAA were applied to A. thaliana Col-0 (wild-type) and tir1.1 (a lateral root deficient mutant) seedlings. Inoculation with heat-inactivated A. argentinense Az39 cells and a non-PGPR bacterium (Escherichia coli DH5α) was also tested. The primary root (PR) length, lateral roots (LR) number, and root hair (HR) density were assessed, and the root transcriptome was sequenced (Illumina HiSeq), followed by DEGs and GO term enrichment analyses. Inoculation with both A. argentinense strains resulted in a shorter PR and an increased number of LR and RH. IAA application (0.1 µM) led to a similar root phenotype than inoculation with Az39 (108 CFU mL−1). The addition of 1 µM flagellin, as well as plant exposure to non-lysed A. argentinense Az39 or E. coli DH5α cells, enhanced RH formation. Genes related to auxin signaling were highly expressed in the roots of Az39-inoculated seedlings; genes related to jasmonate and salicylic acid metabolism were highly expressed in the roots of plants inoculated with ipdC −. Root architecture changes in A. thaliana inoculated with A. argentinense Az39 do not depend exclusively on root IAA levels/IAA plant perception. This PGPR induces root morphological changes through both IAA-dependent and IAA-independent mechanisms. Flagellin may be a key molecule involved in IAA-independent mechanisms.
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Articulos (INIAB)
Articulos de INSTITUTO DE INVESTIGACIONES AGROBIOTECNOLOGICAS
Articulos de INSTITUTO DE INVESTIGACIONES AGROBIOTECNOLOGICAS
Articulos(IIBBA)
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Citación
Mora, Maria Veronica; Lopez, Gaston Alberto; Molina, Romina Micaela; Coniglio, Nayla Anahí; Nievas, Sofia Mariela; et al.; Azospirillum argentinense Modifies Arabidopsis Root Architecture Through Auxin-dependent Pathway and Flagellin; Springer; Journal of Soil Science and Plant Nutrition; 23; 3; 6-2023; 4543-4557
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