Artículo
Development and field evaluation of an ELISA to differentiate Anaplasma marginale–infected from A. centrale–vaccinated cattle
Bellezze, Julio; Thompson, Carolina Soledad; Bosio, Anabela Silvina
; Torioni de Echaide, Susana Marta; Primo, Maria Evangelina
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
Fecha de publicación:
03/2023
Editorial:
American Association of Veterinary Laboratory Diagnosticians
Revista:
Journal of Veterinary Diagnostic Investigation
ISSN:
1040-6387
e-ISSN:
1943-4936
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Immunization of calves with Anaplasma centrale is used to prevent acute anaplasmosis caused by A. marginale. Natural and vaccine-acquired immunity is detected through serologic tests based primarily on A. marginale recombinant major surface protein 5 (MSP5m) because it has 91% identity with MSP5 from A. centrale (MSP5c). We developed a displacement, double-antigen, sandwich ELISA (ddasELISA) to detect antibodies against A. marginale or A. centrale. For ddasELISA validation, we analyzed serum samples positive for antibodies against Anaplasma spp. from cattle naturally infected with A. marginale (n = 300) or vaccinated with A. centrale (n = 255). Species-specific nested PCR (nPCR) assays were used to confirm infection. The optical density (OD) values obtained from antibodies directed at unique epitopes of A. marginale (ODAm) or A. centrale (ODAc) were used in the formula ODAm/ODAc. If the derived ratio was > 0.38, the serum sample was considered positive for antibodies against A. marginale, with 98.9% sensitivity and 98.0% specificity. In a field evaluation, we analyzed 702 Anaplasma spp. antibody–positive serum samples from 34 herds by ddasELISA and nPCR; 571 were classified by ddasELISA as A. marginale–infected or A. centrale–vaccinated, with 84% agreement (κ = 0.70) between ddasELISA and nPCR. Our results indicate that ddasELISA could be used as a cost-effective alternative to molecular techniques to confirm infection with A. marginale in countries in which prevention is based on vaccination with A. centrale.
Palabras clave:
ANAPLASMA CENTRALE
,
ANAPLASMA MARGINALE
,
ELISA
,
SPECIES-SPECIFIC ANTIBODIES
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Licencia
Identificadores
Colecciones
Articulos (IDICAL)
Articulos de INSTITUTO DE INVESTIGACION DE LA CADENA LACTEA
Articulos de INSTITUTO DE INVESTIGACION DE LA CADENA LACTEA
Citación
Bellezze, Julio; Thompson, Carolina Soledad; Bosio, Anabela Silvina; Torioni de Echaide, Susana Marta; Primo, Maria Evangelina; Development and field evaluation of an ELISA to differentiate Anaplasma marginale–infected from A. centrale–vaccinated cattle; American Association of Veterinary Laboratory Diagnosticians; Journal of Veterinary Diagnostic Investigation; 35; 2; 3-2023; 204-208
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