Artículo
Validation of an immunochromatographic assay kit based on colored latex particles for the identification of the canine visceral leishmaniasis
Garcia, Valeria Soledad
; Guerrero, Sergio Adrian
; Burna, Alexis Norberto
; Demonte, Ana; Gugliotta, Luis Marcelino
; González, Verónica Doris Guadalupe
Fecha de publicación:
01/2023
Editorial:
Elsevier
Revista:
Research in Veterinary Science
ISSN:
0034-5288
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Visceral leishmaniasis is a zoonotic infectious disease with a severe impact on humans and animals. Infection is transmitted by phlebotomine sand flies. The dogs are main reservoir for human infection. A rapid and accurate diagnosis of canine visceral leishmaniasis is essential for an efficient surveillance program. The aim of this study was to assess the performance of a rapid immunochromatographic strip test based on functionalized colored particles and a new recombinant antigenic protein, as a visual “in situ” method for the diagnosis of canine visceral leishmaniasis. The results were evaluated using an in-house ELISA assay with the same antigen. Both tests produced concordant results and the immunochromatographic strip test showed good diagnostic sensitivity (98%) and specificity (95%). Finally, meta-analysis was used to compare the sensitivity and specificity of the here developed test with the results of commercial immunochromatographic strip tests obtained from literature.
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Articulos(IAL)
Articulos de INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Articulos de INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Articulos(INTEC)
Articulos de INST.DE DES.TECNOL.PARA LA IND.QUIMICA (I)
Articulos de INST.DE DES.TECNOL.PARA LA IND.QUIMICA (I)
Citación
Garcia, Valeria Soledad; Guerrero, Sergio Adrian; Burna, Alexis Norberto; Demonte, Ana; Gugliotta, Luis Marcelino; et al.; Validation of an immunochromatographic assay kit based on colored latex particles for the identification of the canine visceral leishmaniasis; Elsevier; Research in Veterinary Science; 155; 1-2023; 69-75
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