Mostrar el registro sencillo del ítem
dc.contributor.author
Benavidez, Tomás Enrique
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Guerra, José D. S.
dc.contributor.author
Garcia, Carlos D.
dc.date.available
2023-10-26T12:03:01Z
dc.date.issued
2022-02
dc.identifier.citation
Benavidez, Tomás Enrique; Guerra, José D. S.; Garcia, Carlos D.; Dielectric Spectroscopy can Predict the Effect of External AC Fields on the Dynamic Adsorption of Lysozyme; Wiley VCH Verlag; Chemphyschem; 23; 10; 2-2022; 1-8
dc.identifier.issn
1439-4235
dc.identifier.uri
http://hdl.handle.net/11336/216014
dc.description.abstract
This report describes the application of dielectric spectroscopy as a simple and fast way to guide protein adsorption experiments. Specifically, the polarization behavior of a layer of adsorbed lysozyme was investigated using a triangular-wave signal with frequencies varying from 0.5 to 2 Hz. The basic experiment, which can be performed in less than 5 min and with a single sample, not only allowed confirming the susceptibility of the selected protein towards the electric signal but also identified that this protein would respond more efficiently to signals with lower frequencies. To verify the validity of these observations, the adsorption behavior of lysozyme onto optically transparent carbon electrodes was also investigated under the influence of an applied alternating potential. In these experiments, the applied signal was defined by a sinusoidal wave with an amplitude of 100 mV and superimposed to +800 mV (applied as a working potential) and varying the frequency in the 0.1–10000 Hz range. The experimental data showed that the greatest adsorbed amounts of lysozyme were obtained at the lowest tested frequencies (0.1–1.0 Hz), results that are in line with the corresponding dielectric features of the protein.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley VCH Verlag
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
AC FIELDS
dc.subject
ADSORPTION
dc.subject
CARBON
dc.subject
DIELECTRIC SPECTROSCOPY
dc.subject
LYSOZYME
dc.subject.classification
Físico-Química, Ciencia de los Polímeros, Electroquímica
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
Ciencias Químicas
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.title
Dielectric Spectroscopy can Predict the Effect of External AC Fields on the Dynamic Adsorption of Lysozyme
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-10-25T12:54:13Z
dc.journal.volume
23
dc.journal.number
10
dc.journal.pagination
1-8
dc.journal.pais
Alemania
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.journal.ciudad
Weinheim
dc.description.fil
Fil: Benavidez, Tomás Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
dc.description.fil
Fil: Guerra, José D. S.. Universidade Federal de Uberlandia; Brasil
dc.description.fil
Fil: Garcia, Carlos D.. CLEMSON UNIVERSITY (CLEMSON UNIVERSITY);
dc.journal.title
Chemphyschem
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/cphc.202100914
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/cphc.202100914
Archivos asociados