Artículo
Overexpression of a phosphatidic acid phosphatase type 2 leads to an increase in triacylglycerol production in oleaginous Rhodococcus strains
Hernández, Martín Alejandro
; Comba, Santiago
; Arabolaza, Ana Lorena
; Gramajo, Hugo Cesar
; Alvarez, Hector Manuel
Fecha de publicación:
12/09/2014
Editorial:
Springer
Revista:
Applied Microbiology and Biotechnology
ISSN:
0175-7598
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Oleaginous Rhodococcus strains are able to accumulate large amounts of triacylglycerol (TAG). Phosphatidic acid phosphatase (PAP) enzyme catalyzes the dephosphorylation of phosphatidic acid (PA) to yield diacylglycerol (DAG), a key precursor for TAG biosynthesis. Studies to establish its role in lipid metabolism have been mainly focused in eukaryotes but not in bacteria. In this work, we identified and characterized a putative PAP type 2 (PAP2) encoded by the ro00075 gene in Rhodococcus jostii RHA1. Heterologous expression of ro00075 in Escherichia coli resulted in a fourfold increase in PAP activity and twofold in DAG content. The conditional deletion of ro00075 in RHA1 led to a decrease in the content of DAG and TAG, whereas its overexpression in both RHA1 and Rhodococcus opacus PD630 promoted an increase up to 10 to 15% by cellular dry weight in TAG content. On the other hand, expression of ro00075 in the nonoleaginous strain Rhodococcus fascians F7 promoted an increase in total fatty acid content up to 7% at the expense of free fatty acid (FFA), DAG, and TAG fractions. Moreover, coexpression of ro00075 / atf 2 genes resulted in a fourfold increase in total fatty acid content by a further increase of the FFA and TAG fractions. The results of this study suggest that ro00075 encodes for a PAP2 enzyme actively involved in TAG biosynthesis. Overexpression of this gene, as single one or with an atf gene, provides an alternative approach to increase the biosynthesis and accumulation of bacterial oils as a potential source of raw material for biofuel production.
Palabras clave:
Rhodococcus
,
Triacylglycerols
,
Pap
,
Lipid Metabolism
,
Biofuels
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT-CENPAT)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CENPAT
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CENPAT
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Hernández, Martín Alejandro; Comba, Santiago; Arabolaza, Ana Lorena; Gramajo, Hugo Cesar; Alvarez, Hector Manuel; Overexpression of a phosphatidic acid phosphatase type 2 leads to an increase in triacylglycerol production in oleaginous Rhodococcus strains; Springer; Applied Microbiology and Biotechnology; 99; 5; 12-9-2014; 2191-2207
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