Artículo
Measuring and Perturbing Ferroptosis in Plants
Distefano, Ayelen Mariana
; Marchetti, Maria Fernanda
; Zabaleta, Eduardo Julian
; Pagnussat, Gabriela Carolina
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Fecha de publicación:
05/2022
Editorial:
Humana Press
Revista:
Methods in Molecular Biology
ISSN:
1064-3745
e-ISSN:
1940-6029
ISBN:
978-1-0716-2079-3
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Ferroptosis is an oxidative iron-dependent cell death that was recently described in vertebrates, invertebrates, fungi, plants, and bacteria. In plants, ferroptosis has been reported in response to heat shock in roots of 6-day-old Arabidopsis thaliana seedlings. Generally, all biochemical and morphological ferroptosis hallmarks are conserved between animals and plants. Here, we describe a protocol to induce and quantify ferroptosis in plants based on the analysis of dead cells with a Sytox Green stain. Furthermore, heat shock induced cell death is prevented by using specific ferroptosis inhibitors.
Palabras clave:
CELL DEATH
,
FERROPTOSIS
,
HEAT STRESS
,
PLANTS
,
ROOT HAIRS
,
SYTOX GREEN
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Identificadores
Colecciones
Articulos(IIB)
Articulos de INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Articulos de INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Citación
Distefano, Ayelen Mariana; Marchetti, Maria Fernanda; Zabaleta, Eduardo Julian; Pagnussat, Gabriela Carolina; Measuring and Perturbing Ferroptosis in Plants; Humana Press; Methods in Molecular Biology; 2447; 5-2022; 185-192
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