Artículo
Trace mineral mixture supplemented to in vitro maturation medium improves subsequent embryo development and embryo quality in cattle
Anchordoquy, Juan Patricio
; Balbi, M.; Farnetano, Nicolás Agustín
; Fabra, Mariana Carolina
; Carranza Martin, Ana Cristina
; Nikoloff, Noelia
; Giovambattista, Guillermo
; Furnus, Cecilia Cristina
; Anchordoquy, Juan Mateo
Fecha de publicación:
12/2022
Editorial:
Springer
Revista:
Veterinary Research Communications
ISSN:
0165-7380
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Trace minerals participate in reproductive processes and are crucial for oocyte maturation. The objective of the present study was to investigate the effect of combined supplementation with copper (Cu), manganese (Mn), selenium (Se) and zinc (Zn) during bovine in vitro maturation (IVM) on subsequent embryo development and quality. The IVM medium was supplemented as follows: a) Control (no mineral supplementation); b) MScz (6 ng/mL Mn + 100 ng/mL Se + 200 ng/mL Cu + 400 ng/mL Zn); c) MScZ (6 ng/mL Mn + 100 ng/mL Se + 200 ng/mL Cu + 1200 ng/mL Zn); d) MSCz (6 ng/mL Mn + 100 ng/mL Se + 600 ng/mL Cu + 400 ng/mL Zn). Supplementation with MScz and MSCz produced more blastocysts compared with the control. Total blastocyst cell number was higher when minerals were added at any combination. Day-8 blastocysts derived from oocytes treated with minerals had lower intracellular reactive oxygen species concentration and lipid content than the control. In conclusion, combined supplementation with Cu, Mn, Se and Zn during bovine oocyte IVM increased in vitro production performance, improving embryo developmental ability and quality.
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Articulos(IGEVET)
Articulos de INST.DE GENETICA VET ING FERNANDO NOEL DULOUT
Articulos de INST.DE GENETICA VET ING FERNANDO NOEL DULOUT
Citación
Anchordoquy, Juan Patricio; Balbi, M.; Farnetano, Nicolás Agustín; Fabra, Mariana Carolina; Carranza Martin, Ana Cristina; et al.; Trace mineral mixture supplemented to in vitro maturation medium improves subsequent embryo development and embryo quality in cattle; Springer; Veterinary Research Communications; 46; 4; 12-2022; 1111-1119
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