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dc.contributor.author
Qiao, Ling
dc.contributor.author
Wu, Qi
dc.contributor.author
Lu, Xinya
dc.contributor.author
Zhou, Yan
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Fernández Alvarez, Ana Julia
dc.contributor.author
Ye, Lihong
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Zhang, Xiaodong
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Han, Jihong
dc.contributor.author
Casado, Marta
dc.contributor.author
Liu, Quiang
dc.date.available
2017-07-11T20:55:54Z
dc.date.issued
2013-03
dc.identifier.citation
Qiao, Ling; Wu, Qi; Lu, Xinya; Zhou, Yan; Fernández Alvarez, Ana Julia; et al.; SREBP-1a activation by HBx and the effect on hepatitis B virus enhancer II/core promoter; Academic Press; Biochemical And Biophysical Research Communications; 432; 4; 3-2013; 643-649
dc.identifier.issn
0006-291X
dc.identifier.uri
http://hdl.handle.net/11336/20180
dc.description.abstract
Hepatitis B virus (HBV) X protein (HBx) plays an important role in HBV pathogenesis by regulating gene expression. Sterol regulatory element binding protein-1a (SREBP-1a) is a key transcriptional factor for modulating fatty acid and cholesterol synthesis. Here we demonstrated that HBx increased mature SREBP-1a protein level in the nucleus and its activity as a transcription factor. We further showed that the up-regulation of SREBP-1a by HBx occurred at the transcriptional level after ectopic expression and in the context of HBV replication. Deletional analysis using SREBP-1a promoter revealed that the sequence from -436 to -398 in the promoter was required for its activation by HBx. This promoter region possesses the binding sequences for two basic leucine zipper (b-ZIP) transcription factors, namely C/EBP and E4BP4. Mutagenesis of the binding sequences on the SREBP-1a promoter and ectopic expression experiments demonstrated that C/EBPα enhanced SREBP-1a activation by HBx, while E4BP4 had an inhibitory effect. C/EBPα was able to significantly reverse the inhibitory activity of E4BP4 on SREBP-1a promoter. These results demonstrated that HBx activates SREBP-1a activity at the transcription level through a complex mechanism involving two bZIP transcription factors C/EBP and E4BP4 with C/EBP being the dominant positive factor. Finally, we showed that knocking down SREBP-1 abolishes HBV enhancer II/core promoter activation by HBx.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Academic Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Transcription
dc.subject
Hepatitis
dc.subject
Promoter
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
SREBP-1a activation by HBx and the effect on hepatitis B virus enhancer II/core promoter
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-09-05T13:12:41Z
dc.identifier.eissn
1090-2104
dc.journal.volume
432
dc.journal.number
4
dc.journal.pagination
643-649
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Nueva York
dc.description.fil
Fil: Qiao, Ling. University Of Saskatchewan; Canadá
dc.description.fil
Fil: Wu, Qi. University Of Saskatchewan; Canadá
dc.description.fil
Fil: Lu, Xinya. University Of Saskatchewan; Canadá
dc.description.fil
Fil: Zhou, Yan. University Of Saskatchewan; Canadá
dc.description.fil
Fil: Fernández Alvarez, Ana Julia. Consejo Superior de Investigaciones Cientificas; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
dc.description.fil
Fil: Ye, Lihong. Nankai University; China
dc.description.fil
Fil: Zhang, Xiaodong. Nankai University; China
dc.description.fil
Fil: Han, Jihong. Nankai University; China
dc.description.fil
Fil: Casado, Marta. Consejo Superior de Investigaciones Cientificas; España
dc.description.fil
Fil: Liu, Quiang. University Of Saskatchewan; Canadá
dc.journal.title
Biochemical And Biophysical Research Communications
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0006291X13002775
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.bbrc.2013.02.030
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