Evento
In vivo evaluation of a mixed culture as protector of the intestinal epithelium of BB chicks against the negative effects of soybean agglutinin (SBA)
Babot, Jaime Daniel
; Argañaraz Martínez, Eloy; Apella, Maria Cristina
; Perez Chaia, Adriana Beatriz
Tipo del evento:
Congreso
Nombre del evento:
XI Congreso Argentino de Microbiología General SAMIGE
Fecha del evento:
05/08/2015
Institución Organizadora:
Sociedad Argentina de Microbiología General;
Título del Libro:
Libro de Resúmenes: XI Congreso Argentino de Microbiología General
Editorial:
Sociedad Argentina de Microbiología General
Idioma:
Inglés
Clasificación temática:
Resumen
Lectins, glycoproteins with high resistance to heat, proteolysis and pH, are among the components of the ingredients used for the elaboration of broilers feed. These proteins can specifically and reversibly bind to carbohydrates. Once ingested, they interact with superficial carbohydrates expressed on the surface of intestinal epithelial cells affecting epithelial development and digestive enzyme activities, thus delaying the bird growth. SBA lectin is a secondary metabolite of soybean and specifically binds to N-acetyl-galactosamine and/or galactose. In vitro capture of SBA by Bifidobacterium infantis CRL 1395 was previously reported. Thus, the aim of this study was to evaluate the effect of the administration to BB chicks, fed with a diet supplemented with SBA, of a mixed culture constituted by 5 strains (B. infantis CRL 1395, Enterococcus faecium LET 301, Lactobacillus salivarius LET 201, L. reuteri LET 210 and Propionibacterium acidipropionici LET 103) capable of binding different lectins (SBA, Con A and WGA). Towards this end, a combination of the 5 strains was incorporated into the drinking water (10Exp.6-10Exp.7 CFU/mL, each strain) and daily administered for 13 days to one-day-old BBchicks (group TS, n = 20). A control group (CS, n = 20) including BB chicks fed the same diet but without the bacterial mixture in the drinking water, was also evaluated. All birds were fed a diet supplemented with previously purified SBA to reach approximately 217-354 μg SBA/g of feed. Urea and creatinine in chick´s blood plasma, liver weight/body weight and spleen weight/body weight ratios, bacterial translocation to these organs, and the activity of several digestive enzymes were evaluated at days 6 and 13; jejunal mucosa integrity was studied at day 6. Concerning urea and creatinine in blood plasma, organs/body weight ratios and bacterial translocation to liver and spleen, there were no differences between animals of both groups. All chicks showed alterations in jejunal mucosa, nevertheless birds of group TS had higher overall integrity, showing less immune cells infiltration in lamina propria and no increase in cellularity of the epithelium covering the villi. In concordance to this, mucosa of animals from group TS evidenced significantly higher activities of alkaline phosphatase and leucineaminopeptidase than those of group CS. In conclusion, the administration of the mixed culture prevents some negative effects associated to SBA. Nevertheless, the results of this study indicate that greater protection could be reached through the administration of a higher dose of bifidobacteria in the probiotic mixture.
Palabras clave:
MIXED CULTURE
,
BB CHICKS
,
INTESTINAL EPITELIUM
,
SOYBEAN AGLUTININ
Archivos asociados
Licencia
Identificadores
Colecciones
Eventos(CCT - NOA SUR)
Eventos de CTRO.CIENTIFICO TECNOL.CONICET - NOA SUR
Eventos de CTRO.CIENTIFICO TECNOL.CONICET - NOA SUR
Eventos(CERELA)
Eventos de CENTRO DE REFERENCIA PARA LACTOBACILOS (I)
Eventos de CENTRO DE REFERENCIA PARA LACTOBACILOS (I)
Eventos(PROIMI)
Eventos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Eventos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Citación
In vivo evaluation of a mixed culture as protector of the intestinal epithelium of BB chicks against the negative effects of soybean agglutinin (SBA); XI Congreso Argentino de Microbiología General SAMIGE; Córdoba; Argentina; 2015; 1-1
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