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dc.contributor.author
Sari, Luciana María
dc.contributor.author
Zampini, Renato
dc.contributor.author
Gonzalez del Pino, Francisco
dc.contributor.author
Argañaraz, Martin Eduardo
dc.contributor.author
Ratto, Marcelo Hector
dc.contributor.author
Apichela, Silvana Andrea
dc.date.available
2023-01-11T13:56:51Z
dc.date.issued
2021-01
dc.identifier.citation
Sari, Luciana María; Zampini, Renato; Gonzalez del Pino, Francisco; Argañaraz, Martin Eduardo; Ratto, Marcelo Hector; et al.; Effects of NGF Addition on Llama (Lama glama) Sperm Traits After Cooling; Frontiers Media; Frontiers in Veterinary Science; 7; 1-2021; 1-8
dc.identifier.issn
2297-1769
dc.identifier.uri
http://hdl.handle.net/11336/184307
dc.description.abstract
To provide new insights into the mechanisms through which seminal plasma proteins can protect sperm from damage caused during refrigeration, we evaluate the possibility that β-NGF can contribute to the improvement of sperm quality after cooling. First, β-NGF was detected in refrigerated sperm and compared with unrefrigerated sperm by western blotting of the proteins adsorbed by sperm, showing that native β-NGF is still present even 24 h after cooling only as an active form. Then, the effect of exogenous β-NGF on sperm quality after cooling was evaluated. A total of 12 ejaculates from male llamas (three ejaculates per male), were obtained by electro-ejaculation, diluted 4:1 with buffer Hepes-balanced salt solution and centrifuged at 800 × g for 8 min to remove the seminal plasma. Sperm were suspended in Tris-citrate-fructose-egg yolk diluent for a final concentration of 30 ×106/ml and cooled at 5°C for 24 h. After refrigeration, the extended sperm were equilibrated for 5 min at 37°C and divided into the following subgroups: sperm samples without treatment (control) and sperm samples supplemented with exogenous human β-NGF (10, 100, and 500 ng/ml). At 5, 30, and 60 min of incubation sperm were evaluated for sperm viability (using eosin/nigrosin stain), sperm motility and vigor (observed under light microscopy), and mitochondrial activity (using the JC-1 fluorescent marker). Vigor data were analyzed with the nonparametric Kruskal-Wallis test. The rest of the variables were analyzed with a mixed models approach. Mean comparisons were performed using Fisher's LSD test with a confidence level of 95%. A principal components analysis was performed to analyze the relationships between variables. Treatment of 24 h cooled sperm with 10 or 100 ng/ml of human β-NGF increased the percentage of total motility and vigor (p < 0.05). Besides, an incubation time of 60 min would be adequate to improve sperm quality, since all variables are positively related. The significant improvement observed in the motility and vigor of post-refrigerated sperm suggests that supplementation with exogenous β-NGF may be profitable for the improvement of cooled llama sperm.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Frontiers Media
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
COOLING
dc.subject
CRYOPRESERVATION
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LLAMA (LAMA GLAMA)
dc.subject
SEMINAL PLASMA
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SPERM
dc.subject
Β-NERVE GROWTH FACTOR
dc.subject.classification
Otras Producción Animal y Lechería
dc.subject.classification
Producción Animal y Lechería
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CIENCIAS AGRÍCOLAS
dc.title
Effects of NGF Addition on Llama (Lama glama) Sperm Traits After Cooling
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-04-20T19:31:19Z
dc.journal.volume
7
dc.journal.pagination
1-8
dc.journal.pais
Países Bajos
dc.description.fil
Fil: Sari, Luciana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
dc.description.fil
Fil: Zampini, Renato. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
dc.description.fil
Fil: Gonzalez del Pino, Francisco. Universidad Nacional de Tucumán; Argentina
dc.description.fil
Fil: Argañaraz, Martin Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
dc.description.fil
Fil: Ratto, Marcelo Hector. Universidad Austral de Chile; Chile
dc.description.fil
Fil: Apichela, Silvana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
dc.journal.title
Frontiers in Veterinary Science
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fvets.2020.610597/full
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3389/fvets.2020.610597
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