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dc.contributor.author
Sanllorenti, Pedro Mariano
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dc.contributor.author
Rosenfeld, Jorge
dc.contributor.author
Ronchi, Virginia Paola
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dc.contributor.author
Ferrara, Pascual
dc.contributor.author
Conde, Ruben Danilo
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dc.date.available
2023-01-06T00:30:51Z
dc.date.issued
2001-12
dc.identifier.citation
Sanllorenti, Pedro Mariano; Rosenfeld, Jorge; Ronchi, Virginia Paola; Ferrara, Pascual; Conde, Ruben Danilo; Two dimensional non equilibrium pH gel electrophoresis mapping of cytolic protein changes caused by dietary protein depletion in mouse liver; Springer; Molecular and Cellular Biochemistry; 220; 1-2; 12-2001; 49-56
dc.identifier.issn
0300-8177
dc.identifier.uri
http://hdl.handle.net/11336/183652
dc.description.abstract
Two-dimensional non-equilibrium pH gel electrophoresis (2D-NEPHGE) analysis was used to evaluate the effects of dietary protein depletion on the protein composition of mouse liver cytosol. Analysing the cytosol from both normal and protein depleted liver, the position in gels of more than three hundred protein spots was determined. After 5 days of protein depletion, about 20% of the spots either increased or decreased more than 2 fold. Five spots of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were recognised by specific antibodies. The glutathione S-transferase (GSTs) subunits Yb1, Yc and Yf were identified by the simultaneous analysis of both glutathione-binding cytosolic proteins and the corresponding standards. As estimated by internal optical density (IOD) of spots, the changes caused by protein depletion in GAPDH and GST subunit contents were similar to those obtained by other methods. By means of mass spectrometric analysis of tryptic peptides generated from spots and/or comparison of two-dimensional gel electrophoretic patterns, carbonic anhydrase III (CAIII), Cu, Zn superoxide dismutase (CuZnSOD) and a cytochrome P450 cytosolic protein (cyt P450) were identified. These three proteins, as well as GSTs, are related with intracellular detoxification and free radical scavenging systems. Their contents were regulated by dietary protein restriction in a manner indicative of diminished liver defence against oxidising agents.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CARBONIC ANHYDRASE III
dc.subject
DIETARY PROTEIN DEPLETION
dc.subject
GLUTATHIONE S-TRANSFERASE
dc.subject
GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE
dc.subject
MOUSE LIVER
dc.subject
SUPEROXIDE DISMUTASE
dc.subject.classification
Otras Ciencias Químicas
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dc.subject.classification
Ciencias Químicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Two dimensional non equilibrium pH gel electrophoresis mapping of cytolic protein changes caused by dietary protein depletion in mouse liver
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-12-03T19:57:16Z
dc.journal.volume
220
dc.journal.number
1-2
dc.journal.pagination
49-56
dc.journal.pais
Alemania
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dc.description.fil
Fil: Sanllorenti, Pedro Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
dc.description.fil
Fil: Rosenfeld, Jorge. Laboratoire de Biochimie des Protéines; Francia
dc.description.fil
Fil: Ronchi, Virginia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
dc.description.fil
Fil: Ferrara, Pascual. Laboratoire de Biochimie des Protéines; Francia
dc.description.fil
Fil: Conde, Ruben Danilo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
dc.journal.title
Molecular and Cellular Biochemistry
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1023/A:1010853719232
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1023/a:1010853719232
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