Association between shape changes and bone remodeling patterns in the middle face during ontogeny in South American populations

Abstract

The morphology of facial bones is modeled by processes of bone formation and resorption induced by interactions between tissues and compensatory responses. However, the role of remodeling patterns on the morphological changes within and among populations has been scarcely explored. Here, we assess the association between facial shape and the underlying bone cell activity throughout the ontogeny in two Amerindian populations that represent the extremes of craniofacial variation in South America. The sample comprises 71 individuals (36 adults and 35 subadults) representing hunter-gatherers from Patagonia and horticulturists from Northwest Argentina. We analyzed the shape and size of the zygomatic and the maxilla, and compared them with the patterns of bone formation and resorption. Bone formation and resorption were described by quantitative histological analysis of bone surfaces. Morphological changes were described by landmarks and semilandmarks digitized on 3D surfaces obtained from CT images. The results from multivariate statistics analysis show that the patterns of bone remodeling are associated with variation in the morphology of the middle face. We found a similar pattern of facial shape variation along the ontogenetic trajectory in the two samples, and a similar trend in the amount of formation and resorption activities across ages. The main differences between samples were found in the distribution of the areas of bone formation and resorption, possibly associated with mechanical bone response to masticatory loading. These findings provide clues about the processes and mechanisms of bone development involved in the facial morphological differentiation in human populations from southern South America.