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dc.contributor.author
Cortella, Lucas R. X.
dc.contributor.author
Cestari, Idágene A.
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Lahuerta, Ricardo D.
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Arana, Matheus C.
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Soldera, Marcos Maximiliano
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Rank, Andreas
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Lasagni, Andrés F.
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Cestari, Ismar N.
dc.date.available
2023-01-05T10:23:38Z
dc.date.issued
2021-08
dc.identifier.citation
Cortella, Lucas R. X.; Cestari, Idágene A.; Lahuerta, Ricardo D.; Arana, Matheus C.; Soldera, Marcos Maximiliano; et al.; Conditioning of hiPSC-derived cardiomyocytes using surface topography obtained with high throughput technology; IOP Publishing; Biomedical Materials; 16; 6; 8-2021; 1-39
dc.identifier.issn
1748-6041
dc.identifier.uri
http://hdl.handle.net/11336/183417
dc.description.abstract
Surface functionalization of polymers aims to introduce novel properties that favor bioactive responses. We have investigated the possibility of surface functionalization of polyethylene terephthalate (PET) sheets by the combination of laser ablation with hot embossing and the application of such techniques in the field of stem cell research. We investigated the response of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) to topography in the low micrometer range. HiPSC-CMs are expected to offer new therapeutic tools for myocardial replacement or regeneration after an infarct or other causes of cardiac tissue loss. However, hiPSC-CMs are phenotypically immature compared to myocytes in the adult myocardium, hampering their clinical application. We aimed to develop and test a high-throughput technique for surface structuring that would improve hiPSC-CMs structural maturation. We used laser ablation with a ps-laser source in combination with nanoimprint lithography to fabricate large areas of homogeneous micron- to submicron line-like pattern with a spatial period of 3 µm on the PET surface. We evaluated cell morphology, alignment, sarcomeric myofibrils assembly, and calcium transients to evaluate phenotypic changes associated with culturing hiPSC-CMs on functionalized PET. Surface functionalization through hot embossing was able to generate, at low cost, low micrometer features on the PET surface that influenced the hiPSC-CMs phenotype, suggesting improved structural and functional maturation. This technique may be relevant for high-throughput technologies that require conditioning of hiPSC-CMs and may be useful for the production of these cells for drug screening and disease modeling applications with lower costs.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
IOP Publishing
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CARDIOMYOCYTES
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DIRECT LASER INTERFERENCE PATTERNING
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HIPSC
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POLYETHYLENE TEREPHTHALATE
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ROLL-TO-ROLL NANOIMPRINT LITHOGRAPHY
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SURFACE TOPOGRAPHY
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Bioproductos, Biomateriales, Bioplásticos, Biocombustibles, Bioderivados, etc.
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Biotecnología Industrial
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INGENIERÍAS Y TECNOLOGÍAS
dc.title
Conditioning of hiPSC-derived cardiomyocytes using surface topography obtained with high throughput technology
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-09-29T11:00:29Z
dc.journal.volume
16
dc.journal.number
6
dc.journal.pagination
1-39
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Cortella, Lucas R. X.. Universidade de Sao Paulo; Brasil
dc.description.fil
Fil: Cestari, Idágene A.. Universidade de Sao Paulo; Brasil
dc.description.fil
Fil: Lahuerta, Ricardo D.. Universidade de Sao Paulo; Brasil
dc.description.fil
Fil: Arana, Matheus C.. Universidade de Sao Paulo; Brasil
dc.description.fil
Fil: Soldera, Marcos Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina
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Fil: Rank, Andreas. Technische Universität Dresden; Alemania
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Fil: Lasagni, Andrés F.. Technische Universität Dresden; Alemania
dc.description.fil
Fil: Cestari, Ismar N.. Universidade de Sao Paulo; Brasil
dc.journal.title
Biomedical Materials
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://iopscience.iop.org/article/10.1088/1748-605X/ac1f73
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1088/1748-605X/ac1f73
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