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dc.contributor.author
Sineli, Pedro Eugenio
dc.contributor.author
Aparicio, Juan Daniel
dc.contributor.author
Pernodet, Jean Luc
dc.contributor.author
Polti, Marta Alejandra
dc.date.available
2022-12-21T10:03:42Z
dc.date.issued
2019
dc.identifier.citation
Proteomic analysis to understand Cr(VI) homeostasis in Streptomyces sp. MC1; LV Annual SAIB Meeting and XIV PABMB Congress; Salta; Argentina; 2019; 1-2
dc.identifier.issn
0327-9545
dc.identifier.uri
http://hdl.handle.net/11336/181842
dc.description.abstract
Chromium is a heavy metal widely used in a variety of industrial processes (leather tanning, steel production, metal corrosion inhibition). Hexavalent chromium is carcinogenic and presents higher toxicity than trivalent form since Cr(VI) is more water-soluble and mobile than Cr(III). Industrial effluents containing Cr(VI) are released into water courses, mostly without proper treatment, resulting in anthropogenic contamination. Over the last years, bacteria-mediated removal or stabilization of heavy metal into no or less toxic forms has become in an effective biotechnological process. In this sense, several physiological studies on Streptomyces sp. MC1, an actinobacteria isolated from a polluted soil in the province of Tucumán (Argentina), demonstrated be able to grow in presence of Cr(VI) and remove the metal both in liquid medium and contaminated soils. However, the molecular mechanisms involved are unknown in this actinobacteria. MS-based proteomics have become a powerful tool to understand the mechanisms that underlie physiological processes. In the present work, we use MS-based, label-free and quantitative proteomic analyses in order to identify enzymes involves in oxidative stress response caused by the presence of Cr(VI) in our actinobacteria strain MC1. Sampling points for proteomics analyses were established according to the growth of Streptomyces sp. MC1 in minimal medium (MM) amended with Cr(VI) at 50 mg L-1 and MM without the metal (control condition). Cells were harvested after 18 and 24 h of incubation in control condition and MM with Cr(VI) respectively. These sampling points allowed obtaining comparable and metabolically active cells (exponential phase of growth). Cr(VI) removal was 10% at the time that cells were harvested (24 h). A total of 1981 different proteins were detected in the proteome. It represents approximately 22% of the predicted protein sequences for this strain. 518 of these proteins passed our significance parameters which 186 of them were up-regulated in the condition supplemented with Cr(VI). Analysis with the software BlastKOALA showed that up-regulated proteins were distributed in metabolic pathways that result essential for a correct cellular operation. Overall, the proteins were related to carbon and energy metabolism, genetic information processing, oxidative stress response and membrane transports. Interestingly, enzymes from pentose phosphate pathway increasing significantly their abundance in presence of chromium. About, 10 different oxidoreductases enzymes were up-regulated in presence of the metal. Regarding oxidative stress response, key enzymes like superoxide dismutase, catalase, mycothiol synthase, and mycothiol amidase were identified with an increment in their abundance. The proteome analysis performed in Streptomyces sp. MC1 allowed us to identify the proteins involves in the homeostasis of Cr(VI). These results serve as basement to study and improve the heavy metal removal by actinobacteria.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Tech Science Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
PROTEOMIC
dc.subject
Cr(VI)
dc.subject
HOMEOSTASIS
dc.subject
STREPTOMYCES
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Proteomic analysis to understand Cr(VI) homeostasis in Streptomyces sp. MC1
dc.type
info:eu-repo/semantics/publishedVersion
dc.type
info:eu-repo/semantics/conferenceObject
dc.type
info:ar-repo/semantics/documento de conferencia
dc.date.updated
2022-12-15T11:07:47Z
dc.journal.volume
49
dc.journal.number
Suplemento 1
dc.journal.pagination
1-2
dc.journal.pais
Argentina
dc.journal.ciudad
Mendoza
dc.description.fil
Fil: Sineli, Pedro Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Aparicio, Juan Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Pernodet, Jean Luc. Institut de Biologie Intégrative de la Cellule; Francia
dc.description.fil
Fil: Polti, Marta Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2019.pdf
dc.conicet.rol
Autor
dc.conicet.rol
Autor
dc.conicet.rol
Autor
dc.conicet.rol
Autor
dc.coverage
Internacional
dc.type.subtype
Congreso
dc.description.nombreEvento
LV Annual SAIB Meeting and XIV PABMB Congress
dc.date.evento
2019-11-05
dc.description.ciudadEvento
Salta
dc.description.paisEvento
Argentina
dc.type.publicacion
Journal
dc.description.institucionOrganizadora
Sociedad Argentina de Investigaciones Bioquímicas
dc.source.revista
Biocell
dc.date.eventoHasta
2019-11-08
dc.type
Congreso
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