Evento
Transcriptional and proteomic analyses of redox enviorement in beta-thalassemia trait
Teran, Magdalena María
; Monaco, Maria Eugenia; Sineli, Pedro Eugenio
; Haro, Ana Cecilia
; Lazarte, Sandra Stella; Ledesma Achem, Emilse; Alvarez Asensio, Natalia Sofía
; Agüero Aguilera, Ana Carolina
; Isse, Blanca Alicia de Los Angeles G.
Tipo del evento:
Reunión
Nombre del evento:
LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión Anual de la Sociedad Argentina de Inmunología; LIII Reunión Anual de la Asociación Argentina de Farmacología Experimental y XI Reunión Anual de la Asociación Argentina de Nanomedicinas
Fecha del evento:
17/11/2021
Institución Organizadora:
Sociedad Argentina de Investigación Clínica;
Sociedad Argentina de Inmunología;
Asociación Argentina de Farmacología Experimental;
Asociación Argentina de Nanomedicinas;
Título de la revista:
Medicina (Buenos Aires)
Editorial:
Fundación Revista Medicina
ISSN:
1669-9106
Idioma:
Inglés
Clasificación temática:
Resumen
β-Thalassemia trait (BTT) is a heterogeneous group of genetic defects leading to decreased β -globin production, ineffective erythropoiesis and oxidative stress. Reactive oxygen speciesproduction and oxidative environment have an impact on all blood cell lineages. The aim was toevaluate, at transcriptional and proteomic levels, the pro-oxidative and anti-oxidative status in BTTpatients. A descriptive study was performed with 66 subjects (40 apparently healthy and 26 withBTT). Real-time PCR was used for gene expression analyses of transcription factors forkheadhomeobox typeO (FOXO3a) and nuclear factor erythroid2-related factor-2 (NRF2); antioxidantenzymes: catalase (CAT), peroxiredoxin-2 (PRX-2), superoxide dismutase (SOD); and cytokines TNF-α, IL-6. Quantitative mass spectrometry was performed on cytosol erythrocyte membranesdepleted of hemoglobin. Bioinformatic analysis was performed with Perseus, BlastKoala andProteome Discoverer V1.4 programs.Relative expression of NRF2 was 4.7-fold higher in BTT than in control group, whereas FOXO3aexpression was similar in both. Transcriptional expression of SOD, PRDX2 and proinflammatorycytokines were significantly upregulated in BTT compared to controls (p<0,005). Proteomic studyshowed significant difference in abundances of oxidative stress and inflammation markers such aslipoxygenase15 (ALOX15), poly-C-binding protein 1/2 (PCBP 1/2), P40/P67 subunit of NADPHoxidase and 70 kilodalton heat shock protein (HSP70), tyrosine-protein kinase (SYK) in BTT (p˂0,05). Proteins involved in redox imbalance protection such as glutathione S-transferase kappa1(GSTκ1), isocitrate dehydrogenase 1/2 (IDH1/2) and glucose-6-phosphate dehydrogenase (G6PD)were higher in BTT than in controls (4.2, 4.1 and 2.1 fold respectively). These results showedchanges in the gene expression of some redox regulators together with modifications in theerythrocyte proteome generated by the global redox imbalance underlying in this pathology
Palabras clave:
TALASEMIA
,
PROTEOMA
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Eventos(PROIMI)
Eventos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Eventos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Citación
Transcriptional and proteomic analyses of redox enviorement in beta-thalassemia trait; LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión Anual de la Sociedad Argentina de Inmunología; LIII Reunión Anual de la Asociación Argentina de Farmacología Experimental y XI Reunión Anual de la Asociación Argentina de Nanomedicinas; Ciudad Autónoma de Buenos Aires; Argentina; 2021; 1-5
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