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dc.contributor.author
Larraburu, Ezequiel Enrique
dc.contributor.author
Yarte, Mauro Enrique
dc.contributor.author
Llorente, Berta Elizabet
dc.date.available
2022-11-25T16:24:12Z
dc.date.issued
2016-08
dc.identifier.citation
Larraburu, Ezequiel Enrique; Yarte, Mauro Enrique; Llorente, Berta Elizabet; Azospirillum brasilense inoculation, auxin induction and culture medium composition modify the profile of antioxidant enzymes during in vitro rhizogenesis of pink lapacho; Springer; Plant Cell, Tissue and Organ Culture; 127; 2; 8-2016; 381-392
dc.identifier.issn
0167-6857
dc.identifier.uri
http://hdl.handle.net/11336/179040
dc.description.abstract
Optimal in vitro plant growth can be stimulated by selecting specific nutritional and environmental conditions. However, the culture conditions, dissection, and disinfection of plant material are stressful and may induce disruption of the plant physiological homeostasis. This can be modified by inoculation with rhizobacteria as Azospirillum brasilense, by the culture medium type, and by auxin induction. Here, we performed rooting experiments in two auxin-free culture media with ‘pink lapacho’ (Handroanthus impetiginosus) shoots previously induced with 0, 10, 30, or 50 μM indole butyric acid (IBA) for 3 days and inoculated with A. brasilense Cd and Az39. Peroxidase (PO), phenylalanine ammonia-lyase (PAL), and polyphenol oxidase (PPO) activities were determined on days 0, 3, 6, 9, 12, and 15. Also, weekly absolute rooting percentage was evaluated. All enzymatic activities were higher in A. brasilense-inoculated shoots, linked to early and high rooting percentage. The culture medium type and IBA concentration also affected enzymatic activities. The positive correlation between PO and PAL activities on day 9 and successful final in vitro rooting of H. impetiginosus allows using these activities as early markers of rhizogenesis reducing the selection time of easy-to-root plants. The changes in enzymatic levels performed here are discussed on the basis of their role in rooting and in vitro stress and contribute to the knowledge of the physiology of trees and their interaction with rhizobacteria.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
HANDROANTHUS IMPETIGINOSUS
dc.subject
IN VITRO ROOTING
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PEROXIDASE
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PHENYLALANINE AMMONIA-LYASE
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POLYPHENOL OXIDASE
dc.subject.classification
Otras Biotecnología Agropecuaria
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Biotecnología Agropecuaria
dc.subject.classification
CIENCIAS AGRÍCOLAS
dc.title
Azospirillum brasilense inoculation, auxin induction and culture medium composition modify the profile of antioxidant enzymes during in vitro rhizogenesis of pink lapacho
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-11-25T12:03:00Z
dc.journal.volume
127
dc.journal.number
2
dc.journal.pagination
381-392
dc.journal.pais
Alemania
dc.journal.ciudad
Berlín
dc.description.fil
Fil: Larraburu, Ezequiel Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Lujan. Departamento de Cs.basicas. Laboratorio de Cultivo de Tejidos Vegetales; Argentina
dc.description.fil
Fil: Yarte, Mauro Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Lujan. Departamento de Cs.basicas. Laboratorio de Cultivo de Tejidos Vegetales; Argentina
dc.description.fil
Fil: Llorente, Berta Elizabet. Universidad Nacional de Lujan. Departamento de Cs.basicas. Laboratorio de Cultivo de Tejidos Vegetales; Argentina
dc.journal.title
Plant Cell, Tissue and Organ Culture
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s11240-016-1060-z
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s11240-016-1060-z
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