Artículo
Ultrastructural characterization of the tegument in protoscoleces of Echinococcus ortleppi
Miles, Sebastián; Magnone, Javier; García Luna, Joaquín; Ancarola, María Eugenia
; Cucher, Marcela Alejandra
; Dematteis, Sylvia; Frischknecht, Friedrich; Cyrklaff, Marek; Mourglia Ettlin, Gustavo
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
Fecha de publicación:
11/2021
Editorial:
Elsevier
Revista:
International Journal for Parasitology
ISSN:
0020-7519
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Cystic echinococcosis is a globally distributed zoonosis caused by cestodes of the Echinococcus granulosus sensu lato (s.l.) complex, with Echinococcus ortleppi mainly involved in cattle infection. Protoscoleces show high developmental plasticity, being able to differentiate into either adult worms or metacestodes within definitive or intermediate hosts, respectively. Their outermost cellular layer is called the tegument, which is important in determining the infection outcome through its immunomodulating activities. Herein, we report an in-depth characterization of the tegument of E. ortleppi protoscoleces performed through a combination of scanning and transmission electron microscopy techniques. Using electron tomography, a three-dimensional reconstruction of the tegumental cellular territories was obtained, revealing a novel structure termed the ‘tegumental vesicular body’ (TVB). Vesicle-like structures, possibly involved in endocytic/exocytic routes, were found within the TVB as well as in the parasite glycocalyx, distal cytoplasm and close inner structures. Furthermore, parasite antigens (GST-1 and AgB) were unevenly localised within tegumental structures, with both being detected in vesicles found within the TBV. Finally, the presence of host (bovine) IgG was also assessed, suggesting a possible endocytic route in protoscoleces. Our data forms the basis for a better understanding of E. ortleppi and E. granulosus s.l. structural biology.
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Articulos(IMPAM)
Articulos de INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Articulos de INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Citación
Miles, Sebastián; Magnone, Javier; García Luna, Joaquín; Ancarola, María Eugenia; Cucher, Marcela Alejandra; et al.; Ultrastructural characterization of the tegument in protoscoleces of Echinococcus ortleppi; Elsevier; International Journal for Parasitology; 51; 12; 11-2021; 989-997
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