Evento
Purification of anti-RBD antibodies obtained from llama (Lama glama) for potential Covid-19 therapy
Soliz Santander, Silvana Estafanía
; Stagnetto, Agustín
; Ploper, Diego
; Tomas Grau, Rodrigo Hernán
; Avila, Cesar Luis
; Cazorla, Silvia Ines
; Maldonado Galdeano, María Carolina
; Argañaraz, Martin Eduardo
; Apichela, Silvana Andrea
; Perdigon, Gabriela del Valle
; Chehin, Rosana Nieves
; Vera Pingitore, Esteban
Tipo del evento:
Reunión
Nombre del evento:
XXXVIII Annual Scientific Meeting
Fecha del evento:
20/10/2021
Institución Organizadora:
Asociación de Biología de Tucumán;
Título de la revista:
Biocell
Editorial:
Tech Science Press
ISSN:
0327-9545
e-ISSN:
1667-5746
Idioma:
Inglés
Clasificación temática:
Resumen
Despite worldwide efforts to develop different treatments for SARS-CoV-2 COVID-19, the situation remains critical, requiring rapid and effective strategies. In this regard, antibodies (Ab) have demonstrated clinical potential. Among them, camelid nanoAb (VHH) arise as a possible alternative, as they recognize epitopes which are inaccessible to conventional Ab. Further advantages of VHH are their small size, high solubility, high stability and resistance to low pH. The aim of this work is to describe a purification scheme of different isotypes of anti-SARS-CoV-2 immunoglobulin G (IgG) produced after immunizing two llamas (Lama glama). To achieve this, plasma was injected into an affinity chromatographic column (Protein G), and the resulting fractions were analyzed by SDS-PAGE under non-reducing conditions. The anti-RBD titers were determined by an "in house" ELISA, reaching titers of 52000 and 13000 for IgG1 and IgG3 fractions, respectively. Subsequently, an affinity column (HiTrap NHS-activated) was prepared to separate monospecific anti-RBD polyclonal Ab. RBD produced in our laboratory was covalently coupled to this column, achieving a coupling efficiency of 97%. Different isotypes of monospecific anti-RBD Ab (IgG1140kDa e IgG395kDa) were obtained. IgG3 represent the starting point for obtaining VHH and/or evaluating their potential use as a therapeutic or preventive alternative, which represents a notable regional contribution in the fight against COVID-19.
Palabras clave:
ANTI-RBD
,
LLAMA
,
NANO ANTICUERPOS
,
SARS-COV-2
,
COVID-19
Archivos asociados
Licencia
Identificadores
Colecciones
Eventos (IMMCA)
Eventos de INSTITUTO DE INVESTIGACIONES EN MEDICINA MOLECULAR Y CELULAR APLICADA DEL BICENTENARIO
Eventos de INSTITUTO DE INVESTIGACIONES EN MEDICINA MOLECULAR Y CELULAR APLICADA DEL BICENTENARIO
Eventos(CERELA)
Eventos de CENTRO DE REFERENCIA PARA LACTOBACILOS (I)
Eventos de CENTRO DE REFERENCIA PARA LACTOBACILOS (I)
Eventos(INSIBIO)
Eventos de INST.SUP.DE INVEST.BIOLOGICAS
Eventos de INST.SUP.DE INVEST.BIOLOGICAS
Citación
Purification of anti-RBD antibodies obtained from llama (Lama glama) for potential Covid-19 therapy; XXXVIII Annual Scientific Meeting ; Tucumán; Argentina; 2021; 1-3
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