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dc.contributor.author
Yeo, Matthew
dc.contributor.author
Mauricio, Isabel L.
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Messenger, Loisa A.
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Lewis, Michael D.
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Llewellyn, Martin S.
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Acosta, Nidia
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Bhattacharyya, Tapan
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Diosque, Patricio
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Carrasco, Hernán J.
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Miles, Michael A.
dc.date.available
2017-05-22T19:12:35Z
dc.date.issued
2011-06-21
dc.identifier.citation
Yeo, Matthew; Mauricio, Isabel L.; Messenger, Loisa A.; Lewis, Michael D.; Llewellyn, Martin S.; et al.; Multilocus Sequence Typing (MLST) for Lineage Assignment and High Resolution Diversity Studies in Trypanosoma cruzi; Public Library Of Science; Neglected Tropical Diseases; 5; 6; 21-6-2011; 1049-1049
dc.identifier.issn
1935-2735
dc.identifier.uri
http://hdl.handle.net/11336/16813
dc.description.abstract
PLoS Negl Trop Dis. 2011 Jun;5(6):e1049. Epub 2011 Jun 21. Multilocus sequence typing (MLST) for lineage assignment and high resolution diversity studies in Trypanosoma cruzi. Yeo M, Mauricio IL, Messenger LA, Lewis MD, Llewellyn MS, Acosta N, Bhattacharyya T, Diosque P, Carrasco HJ, Miles MA. Source London School of Hygiene and Tropical Medicine, London, United Kingdom. Matthew.yeo@lshtm.ac.uk Abstract BACKGROUND: Multilocus sequence typing (MLST) is a powerful and highly discriminatory method for analysing pathogen population structure and epidemiology. Trypanosoma cruzi, the protozoan agent of American trypanosomiasis (Chagas disease), has remarkable genetic and ecological diversity. A standardised MLST protocol that is suitable for assignment of T. cruzi isolates to genetic lineage and for higher resolution diversity studies has not been developed. METHODOLOGY/PRINCIPAL FINDINGS: We have sequenced and diplotyped nine single copy housekeeping genes and assessed their value as part of a systematic MLST scheme for T. cruzi. A minimum panel of four MLST targets (Met-III, RB19, TcGPXII, and DHFR-TS) was shown to provide unambiguous assignment of isolates to the six known T. cruzi lineages (Discrete Typing Units, DTUs TcI-TcVI). In addition, we recommend six MLST targets (Met-II, Met-III, RB19, TcMPX, DHFR-TS, and TR) for more in depth diversity studies on the basis that diploid sequence typing (DST) with this expanded panel distinguished 38 out of 39 reference isolates. Phylogenetic analysis implies a subdivision between North and South American TcIV isolates. Single Nucleotide Polymorphism (SNP) data revealed high levels of heterozygosity among DTUs TcI, TcIII, TcIV and, for three targets, putative corresponding homozygous and heterozygous loci within DTUs TcI and TcIII. Furthermore, individual gene trees gave incongruent topologies at inter- and intra-DTU levels, inconsistent with a model of strict clonality. CONCLUSIONS/SIGNIFICANCE: We demonstrate the value of systematic MLST diplotyping for describing inter-DTU relationships and for higher resolution diversity studies of T. cruzi, including presence of recombination events. The high levels of heterozygosity will facilitate future population genetics analysis based on MLST haplotypes.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library Of Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Mlst
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Typing
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Trypanosoma Cruzi
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Parasitología
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Ciencias de la Salud
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Multilocus Sequence Typing (MLST) for Lineage Assignment and High Resolution Diversity Studies in Trypanosoma cruzi
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-05-02T18:16:23Z
dc.journal.volume
5
dc.journal.number
6
dc.journal.pagination
1049-1049
dc.journal.pais
Estados Unidos
dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Yeo, Matthew. London School of Hygiene and Tropical Medicine; Reino Unido
dc.description.fil
Fil: Mauricio, Isabel L.. Instituto de Higiene e Medicina Tropical; Portugal
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Fil: Messenger, Loisa A.. London School of Hygiene and Tropical Medicine; Reino Unido
dc.description.fil
Fil: Lewis, Michael D.. London School of Hygiene and Tropical Medicine; Reino Unido
dc.description.fil
Fil: Llewellyn, Martin S.. London School of Hygiene and Tropical Medicine; Reino Unido
dc.description.fil
Fil: Acosta, Nidia. London School of Hygiene and Tropical Medicine; Reino Unido. Universidad Nacional de Asuncion; Paraguay
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Fil: Bhattacharyya, Tapan. London School of Hygiene and Tropical Medicine; Reino Unido
dc.description.fil
Fil: Diosque, Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Cs.de la Salud. Instituto de Patología Experimental; Argentina
dc.description.fil
Fil: Carrasco, Hernán J.. Universidad Central de Venezuela; Venezuela
dc.description.fil
Fil: Miles, Michael A.. London School of Hygiene and Tropical Medicine; Reino Unido
dc.journal.title
Neglected Tropical Diseases
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0001049
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pntd.0001049
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