Artículo
Exogenous activation and inhibition of plasminogen/plasmin activity during in vitro maturation of bovine cumulus-oocyte complexes: A biological and spectroscopic approach
Rizo, Gabriela
; Barrera, Antonio Daniel
; Jimenez, Luis Emanuel
; Garcia, Elina Vanesa
; Garcia, Daniela Celeste
; Roldan Olarte, Eugenia Mariela
Fecha de publicación:
11/2020
Editorial:
Wiley-liss, div John Wiley & Sons Inc.
Revista:
Molecular Reproduction and Development
ISSN:
1040-452X
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
This study deals with the effect of plasminogen/plasmin on the in vitro maturation (IVM) of bovine cumulus-oocyte complexes (COCs). Exogenous plasminogen activator streptokinase (SK) added to the IVM medium revealed similar values of cumulus expansion and oocyte nuclear maturation compared to controls (standard IVM medium). However, a decrease in both determinations was observed in COCs matured with the supplementation of ɛ-aminocaproic acid (ɛ-ACA), a specific plasmin inhibitor. After in vitro fertilization, no differences were observed in either cleavage or blastocyst rates between SK and control groups; however, ε-ACA treatment caused a decrease in both developmental rates. Zona pellucida (ZP) digestion time decreased in the SK group while it increased in the ε-ACA group. Raman microspectroscopy revealed an increase in the intensity of the band corresponding to the glycerol group of sialic acid in the ZP of oocytes matured with SK, whereas ZP spectra of oocytes treated with ɛ-ACA presented similarities with immature oocytes. The results indicate that although treatment with SK did not alter oocyte developmental competence, it induced modifications in the ZP of oocytes that could modify the folding of glycoproteins. Plasmin inhibition impairs oocyte maturation and has an impact on embryo development, thus evidencing the importance of this protease during IVM.
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(INSIBIO)
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Citación
Rizo, Gabriela; Barrera, Antonio Daniel; Jimenez, Luis Emanuel; Garcia, Elina Vanesa; Garcia, Daniela Celeste; et al.; Exogenous activation and inhibition of plasminogen/plasmin activity during in vitro maturation of bovine cumulus-oocyte complexes: A biological and spectroscopic approach; Wiley-liss, div John Wiley & Sons Inc.; Molecular Reproduction and Development; 88; 1; 11-2020; 67-79
Compartir
Altmétricas