Nitric oxide and membrane lipid peroxidation in photosynthetic and non-photosynthetic organisms under several stress conditions

Abstract

Oxidative damage to lipids was characterized in terms of the nature of the oxidant, the type of lipid, and the severity of the oxidation (Simontacchi et al., 2011). Even though malondialdehyde detection with the thiobarbituric acid reactive substances test (TBARS) is the most currently used assay for the determination of lipid oxidation, it is unspecific since the reaction can be reproduced by other biological compounds (Simontacchi et al., 2011). On the other hand, electron paramagnetic resonance (EPR) spectroscopy showed the capacity of detecting the presence of the lipid radicals (LR•) formed during peroxidation, by yielding unique and stable products with spin traps (Malanga and Puntarulo, 2012)...