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dc.contributor.author
Centi, Sonia
dc.contributor.author
Messina, Germán Alejandro
dc.contributor.author
Trombelli, Sara
dc.contributor.author
Palchetti, Ilaria
dc.contributor.author
Mascini, Marco
dc.date.available
2022-05-27T19:43:53Z
dc.date.issued
2008-06
dc.identifier.citation
Centi, Sonia; Messina, Germán Alejandro; Trombelli, Sara; Palchetti, Ilaria; Mascini, Marco; Different approaches for the detection of thrombin by an electrochemical aptamer-based assay coupled to magnetic beads; Elsevier Advanced Technology; Biosensors & Bioelectronics; 23; 11; 6-2008; 1602-1609
dc.identifier.issn
0956-5663
dc.identifier.uri
http://hdl.handle.net/11336/158404
dc.description.abstract
Different assay formats based on the coupling of magnetic beads with electrochemical transduction were compared here for the detection of thrombin by using a thrombin specific aptamer. By using the thrombin-binding aptamer, a direct and an indirect competitive assay for thrombin have been developed by immobilising the aptamer or the protein, respectively. Moreover, another strategy was based on the direct measurement of the enzymatic product of thrombin captured by the immobilised aptamer. All the assays were developed by coupling the electrochemical transduction with the innovative and advantageous use of magnetic beads. The assays based on the immobilisation of the protein were not successful since no binding was recorded between thrombin and its aptamer. With the direct competitive assay, when the aptamer was immobilised onto the magnetic beads, a detection limit of 430 nM for thrombin was achieved. A lower detection limit for the protein (175 nM) was instead obtained by detecting the product of the enzymatic reaction catalysed by thrombin. All these assays were finally compared with a sandwich assay which reached a detection limit of 0.45 nM of thrombin demonstrating the best analytical performances. With this comparison the importance of a deep study on the different analytical approaches for thrombin detection to reach the performances of the best assay configuration has been demonstrated.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Advanced Technology
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
APTAMER
dc.subject
THROMBIN
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MAGNETIC BEADS
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ELECTROCHEMICAL DETECTION
dc.subject.classification
Química Analítica
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Different approaches for the detection of thrombin by an electrochemical aptamer-based assay coupled to magnetic beads
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-05-20T15:07:17Z
dc.journal.volume
23
dc.journal.number
11
dc.journal.pagination
1602-1609
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Centi, Sonia. Università degli Studi di Firenze; Italia
dc.description.fil
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Università degli Studi di Firenze; Italia
dc.description.fil
Fil: Trombelli, Sara. Università degli Studi di Firenze; Italia
dc.description.fil
Fil: Palchetti, Ilaria. Università degli Studi di Firenze; Italia
dc.description.fil
Fil: Mascini, Marco. Università degli Studi di Firenze; Italia
dc.journal.title
Biosensors & Bioelectronics
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0956566308000390
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.bios.2008.01.020
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