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dc.contributor.author
Valladares, Ana
dc.contributor.author
Rodríguez, Virginia
dc.contributor.author
Camargo, Sergio
dc.contributor.author
Martínez Noël, Giselle María Astrid
dc.contributor.author
Herrero, Antonia
dc.contributor.author
Luque, Ignacio
dc.date.available
2017-04-25T15:26:37Z
dc.date.issued
2011-03
dc.identifier.citation
Valladares, Ana; Rodríguez, Virginia; Camargo, Sergio; Martínez Noël, Giselle María Astrid; Herrero, Antonia; et al.; A specific role of the cyanobacterial PipX factor in the heterocysts of Anabaena sp. strain PCC 7120; American Society For Microbiology; Journal Of Bacteriology; 193; 5; 3-2011; 1172-1182
dc.identifier.issn
0021-9193
dc.identifier.uri
http://hdl.handle.net/11336/15707
dc.description.abstract
The PipX factor is a regulatory protein that seems to occur only in cyanobacteria. In the filamentous, heterocyst-forming Anabaena sp. strain PCC 7120, open reading frame (ORF) asr0485, identified as the pipX gene, is expressed mainly under conditions of combined-nitrogen deprivation dependent on the global N regulator NtcA and the heterocyst-specific regulator HetR. Primer extension and 5′ rapid amplification of cDNA ends (RACE) analyses detected three transcription start points corresponding to a canonical NtcA-activated promoter (to which direct binding of NtcA was observed), an NtcA- and HetR-dependent promoter, and a consensus-type promoter, the last with putative −35 and −10 determinants. Activation of pipX took place in cells differentiating into heterocysts at intermediate to late stages of the process. Accordingly, disruption of pipX led to impaired diazotrophic growth, reduced nitrogenase activity, and impaired activation of the nitrogenase structural genes. The nitrogenase activity of the mutant was low under oxic conditions, likely resulting from inefficient protection against oxygen. In line with this, the activation of the coxB2A2C2 and coxB3A3C3 operons, encoding heterocyst-specific terminal respiratory oxidases responsible for internal oxygen removal, was deficient in the pipX mutant. Therefore, the Anabaena PipX factor shows a spatiotemporal specificity contributing to normal heterocyst function, including full activation of the nitrogenase structural genes and genes of the nitrogenase-protective features of the heterocyst.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society For Microbiology
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Pipx
dc.subject
Anabaena
dc.subject
Heterocyst Function
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
A specific role of the cyanobacterial PipX factor in the heterocysts of Anabaena sp. strain PCC 7120
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-04-24T20:42:30Z
dc.journal.volume
193
dc.journal.number
5
dc.journal.pagination
1172-1182
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Valladares, Ana. Consejo Superior de Investigaciones Cientificas; España. Universidad de Sevilla; España
dc.description.fil
Fil: Rodríguez, Virginia. Consejo Superior de Investigaciones Cientificas; España. Universidad de Sevilla; España
dc.description.fil
Fil: Camargo, Sergio. Consejo Superior de Investigaciones Cientificas; España. Universidad de Sevilla; España
dc.description.fil
Fil: Martínez Noël, Giselle María Astrid. Consejo Superior de Investigaciones Cientificas; España. Universidad de Sevilla; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina
dc.description.fil
Fil: Herrero, Antonia. Consejo Superior de Investigaciones Cientificas; España. Universidad de Sevilla; España
dc.description.fil
Fil: Luque, Ignacio. Consejo Superior de Investigaciones Cientificas; España. Universidad de Sevilla; España
dc.journal.title
Journal Of Bacteriology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://jb.asm.org/content/193/5/1172.short
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1128/JB.01202-10
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