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dc.contributor.author
San Eufrasio, Bonoso
dc.contributor.author
Bigatton, Ezequiel Darío
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dc.contributor.author
Guerrero Sánchez, Victor M.
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dc.contributor.author
Chaturvedi, Palak
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dc.contributor.author
Jorrín Novo, Jesús V.
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dc.contributor.author
Rey, María Dolores
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dc.contributor.author
Castillejo Sánchez, María Ángeles
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dc.date.available
2022-02-11T18:42:51Z
dc.date.issued
2021-03
dc.identifier.citation
San Eufrasio, Bonoso; Bigatton, Ezequiel Darío; Guerrero Sánchez, Victor M.; Chaturvedi, Palak; Jorrín Novo, Jesús V.; et al.; Proteomics Data Analysis for the Identification of Proteins and Derived Proteotypic Peptides of Potential Use as Putative Drought Tolerance Markers for Quercus ilex; MDPI; International Journal of Molecular Sciences; 22; 6; 3-2021; 1-17
dc.identifier.issn
1661-6596
dc.identifier.uri
http://hdl.handle.net/11336/151896
dc.description.abstract
Drought is one of the main causes of mortality in holm oak (Quercus ilex) seedlings used in reforestation programs. Although this species shows high adaptability to the extreme climate conditions prevailing in Southern Spain, its intrinsic genetic variability may play a role in the differential response of some populations and individuals. The aim of this work was to identify proteins and derived proteotypic peptides potentially useful as putative markers for drought tolerance in holm oak by using a targeted post-acquisition proteomics approach. For this purpose, we used a set of proteins identified by shotgun (LC-MSMS) analysis in a drought experiment on Q. ilex seedlings from four different provenances (viz. the Andalusian provinces Granada, Huelva, Cadiz and Seville). A double strategy involving the quantification of proteins and target peptides by shotgun analysis and post-acquisition data analysis based on proteotypic peptides was used. To this end, an initial list of proteotypic peptides from proteins highly represented under drought conditions was compiled that was used in combination with the raw files from the shotgun experiment to quantify the relative abundance of the fragment’s ion peaks with the software Skyline. The most abundant peptides under drought conditions in at least two populations were selected as putative markers of drought tolerance. A total of 30 proteins and 46 derived peptides belonging to the redox, stress-related, synthesis,-folding and degradation, and primary and secondary metabolism functional groups were thus identified. Two proteins (viz., subtilisin and chaperone GrpE protein) were found at increased levels in three populations, which make them especially interesting for validation drought tolerance markers in subsequent experiments.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
MDPI
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
DROUGHT TOLERANCE
dc.subject
PEPTIDE MARKERS
dc.subject
QUERCUS ILEX
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TARGETED POST-ACQUISITION PROTEOMICS
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Proteomics Data Analysis for the Identification of Proteins and Derived Proteotypic Peptides of Potential Use as Putative Drought Tolerance Markers for Quercus ilex
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-02-09T14:21:04Z
dc.identifier.eissn
1422-0067
dc.journal.volume
22
dc.journal.number
6
dc.journal.pagination
1-17
dc.journal.pais
Suiza
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dc.journal.ciudad
Basilea
dc.description.fil
Fil: San Eufrasio, Bonoso. Universidad de Córdoba; España
dc.description.fil
Fil: Bigatton, Ezequiel Darío. Universidad de Córdoba; España. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Departamento de Recursos Naturales. Cátedra de Microbiología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
dc.description.fil
Fil: Guerrero Sánchez, Victor M.. Universidad de Córdoba; España
dc.description.fil
Fil: Chaturvedi, Palak. Vienna University of Technology; Austria
dc.description.fil
Fil: Jorrín Novo, Jesús V.. Universidad de Córdoba; España
dc.description.fil
Fil: Rey, María Dolores. Universidad de Córdoba; España
dc.description.fil
Fil: Castillejo Sánchez, María Ángeles. Universidad de Córdoba; España
dc.journal.title
International Journal of Molecular Sciences
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/22/6/3191
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/ijms22063191
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