Artículo
Dopamine modifies oxygen consumption and mitochondrial membrane potential in striatal mitochondria
Fecha de publicación:
04/2010
Editorial:
Springer
Revista:
Molecular and Cellular Biochemistry
ISSN:
0300-8177
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Dopamine is a neurotransmitter that has been related to mitochondrial dysfunction. In this study, striatal intact mitochondria and submitochondrial membranes were incubated with different dopamine concentrations, and changes on mitochondrial function, hydrogen peroxide, and nitric oxide production were evaluated. A 35% decrease in state 3 oxygen uptake (active respiration state) was found after 1 mM dopamine incubation. In addition, mitochondrial respiratory control significantly decreased, indicating mitochondrial dysfunction. High dopamine concentrations induced mitochondrial depolarization. Also, evaluation of hydrogen peroxide production by intact striatal mitochondria showed a significant increase after 0.5 and 1 mM dopamine incubation. Incubation with 0.5 and 1 mM dopamine increased nitric oxide production in submitochondrial membranes by 28 and 49%, respectively, as compared with control values. This study provides evidence that high dopamine concentrations induce striatal mitochondrial dysfunction through a decrease in mitochondrial respiratory control and loss of membrane potential, probably mediated by free radical production.
Palabras clave:
DOPAMINE
,
MEMBRANE POTENTIAL
,
STRIATAL MITOCHONDRIA
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IBIMOL)
Articulos de INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR
Articulos de INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR
Articulos(OCA HOUSSAY)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Citación
Czerniczyniec, Analia; Bustamante, Joanita; Lores Arnaiz, Silvia; Dopamine modifies oxygen consumption and mitochondrial membrane potential in striatal mitochondria; Springer; Molecular and Cellular Biochemistry; 341; 1-2; 4-2010; 251-257
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