Mostrar el registro sencillo del ítem
dc.contributor.author
Rozés Salvador, María Victoria
dc.contributor.author
Wilson Rodriguez, Carlos
dc.contributor.author
Olmos, Cristina
dc.contributor.author
Gonzalez Billault, Christian
dc.contributor.author
Conde, Cecilia Beatriz
dc.date.available
2021-09-13T16:37:53Z
dc.date.issued
2020-09
dc.identifier.citation
Rozés Salvador, María Victoria; Wilson Rodriguez, Carlos; Olmos, Cristina; Gonzalez Billault, Christian; Conde, Cecilia Beatriz; Fine-Tuning the TGFβ Signaling Pathway by SARA During Neuronal Development; Frontiers Media S.A.; Frontiers in Cell and Developmental Biology; 8; 9-2020; 1-13
dc.identifier.issn
2296-634X
dc.identifier.uri
http://hdl.handle.net/11336/140206
dc.description.abstract
Neural development is a complex process that involves critical events, including cytoskeleton dynamics and selective trafficking of proteins to defined cellular destinations. In this regard, Smad Anchor for Receptor Activation (SARA) is an early endosome resident protein, where perform trafficking- associated functions. In addition, SARA is also involved in cell signaling, including the TGFβ-dependent pathway. Accordingly, SARA, and TGFβ signaling are required for proper axonal specification and migration of cortical neurons, unveiling a critical role for neuronal development. However, the cooperative action between the TGFβ pathway and SARA to this process has remained understudied. In this work, we show novel evidence suggesting a cross-talk between SARA and TGFβ pathway needed for proper polarization, axonal specification, growth and cortical migration of central neurons both in vitro and in vivo. Using microscopy tools and cultured hippocampal neurons, we show a local interaction between SARA and TβRI (TGFβ I receptor) at endosomes. In addition, SARA loss of function, induced by the expression of the dominant-negative SARA-F728A, over-activates the TGFβ pathway, most likely by preserving phosphorylated TβRI. Consequently, SARA-mediated activation of TGFβ pathway impacts on neuronal development, promoting axonal growth and cortical migration of neurons during brain development. Moreover, our data suggests that SARA basally prevents the activation of TβRI through the recruitment of the inhibitory complex PP1c/GADD34 in polarizing neurons. Together, these results propose that SARA is a negative regulator of the TGFβ pathway, being critical for a proper orchestration for neuronal development.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Frontiers Media S.A.
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
AXON
dc.subject
DEVELOPMENT
dc.subject
ENDOSOMES
dc.subject
MIGRATION
dc.subject
NEURONS
dc.subject
SMAD ANCHOR FOR RECEPTOR ACTIVATION (SARA)
dc.subject
TGFΒ
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Fine-Tuning the TGFβ Signaling Pathway by SARA During Neuronal Development
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-08-25T19:28:26Z
dc.journal.volume
8
dc.journal.pagination
1-13
dc.journal.pais
Suiza
dc.description.fil
Fil: Rozés Salvador, María Victoria. Universidad Nacional de Villa María. Instituto Académico Pedagógico de Ciencias Básicas y Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
dc.description.fil
Fil: Wilson Rodriguez, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Instituto Universitario de Ciencias Biomédicas de Córdoba; Argentina
dc.description.fil
Fil: Olmos, Cristina. Universidad de Chile; Chile
dc.description.fil
Fil: Gonzalez Billault, Christian. Universidad de Chile; Chile
dc.description.fil
Fil: Conde, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
dc.journal.title
Frontiers in Cell and Developmental Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.3389/fcell.2020.550267
Archivos asociados