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dc.contributor.author
Ostera, Juan Manuel
dc.contributor.author
Malanga, Gabriela Fabiana
dc.contributor.author
Puntarulo, Susana Ángela
dc.date.available
2021-08-31T15:22:38Z
dc.date.issued
2020-06
dc.identifier.citation
Ostera, Juan Manuel; Malanga, Gabriela Fabiana; Puntarulo, Susana Ángela; Assessment of oxidative balance in hydrophilic cellular environment in Chlorella vulgaris exposed to glyphosate; Pergamon-Elsevier Science Ltd; Chemosphere; 248; 6-2020; 1-8
dc.identifier.issn
0045-6535
dc.identifier.uri
http://hdl.handle.net/11336/139316
dc.description.abstract
The studied hypothesis is that glyphosate (GLY) can affect the oxidative balance in the hydrophilic intracellular medium in non-target Chlorella vulgaris cells. Analytical GLY (5 μM) and a commercial product (RUP) (5 μM) supplementation, did not affect the growth profile. Neither in latent (Lag) nor in exponential (Exp) phase of development, there were significant differences in the cellular abundance, evaluated as cell number, after the supplementation with GLY or RUP. The ascorbyl (A•) content was significantly increased in the presence of GLY or RUP, in Lag and Exp phase of growth. No changes were observed in stationary (St) phase after supplementation with either GLY or RUP. Ascorbate (AH−) content was decreased by 30% in Exp phase of development the presence of RUP. In St phase of the development both, the administration of either GLY or RUP decreased the antioxidant content by 34 and 37%, respectively. The supplementation with GLY and RUP lead to a significant 5- and 10-fold increase in Exp phase, respectively in the A•/AH-content ratio, assessed as a damage/protection ratio in the hydrophilic fraction of the cells, as compared to controls. Neither GLY nor RUP affected the ratio in cells in St phase of development. The data presented here showed experimental evidence that suggested that oxidative balance in the hydrophilic environment is affected by GLY, even at the low to medium concentrations currently used. The effect seems as reversible either because of the magnitude of the herbicide-dependent damage or the antioxidant activity activated.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Pergamon-Elsevier Science Ltd
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ANTIOXIDANT
dc.subject
ASCORBYL/ASCORBATE RATIO
dc.subject
CHLORELLA VULGARIS
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GLYPHOSATE
dc.subject
OXIDATIVE STRESS
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Assessment of oxidative balance in hydrophilic cellular environment in Chlorella vulgaris exposed to glyphosate
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-08-25T19:44:00Z
dc.journal.volume
248
dc.journal.pagination
1-8
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Ostera, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
dc.description.fil
Fil: Malanga, Gabriela Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
dc.description.fil
Fil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
dc.journal.title
Chemosphere
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0045653520301478
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.chemosphere.2020.125955
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