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dc.contributor.author
Tillmann, Urban
dc.contributor.author
Gottschling, Marc
dc.contributor.author
Krock, Bernd
dc.contributor.author
Smith, Kirsty F.
dc.contributor.author
Guinder, Valeria Ana
dc.date.available
2021-06-24T12:42:46Z
dc.date.issued
2019-04
dc.identifier.citation
Tillmann, Urban; Gottschling, Marc; Krock, Bernd; Smith, Kirsty F.; Guinder, Valeria Ana; High abundance of Amphidomataceae (Dinophyceae) during the 2015 spring bloom of the Argentinean Shelf and a new, non-toxigenic ribotype of Azadinium spinosum; Elsevier Science; Harmful Algae; 84; 4-2019; 244-260
dc.identifier.issn
1568-9883
dc.identifier.uri
http://hdl.handle.net/11336/134839
dc.description.abstract
Azaspiracids (AZA) are the most recently discovered group of lipophilic marine biotoxins of microalgal origin, and associated with human incidents of shellfish poisoning. They are produced by a few species of Amphidomataceae, but diversity and occurrence of the small-sized dinophytes remain poorly explored for many regions of the world. In order to analyze the presence and importance of Amphidomataceae in a highly productive area of Argentinean coastal waters (El Rincón area, SW Atlantic), a scientific cruise was performed in 2015 to sample the early spring bloom. In a multi-method approach, light microscopy was combined with real-time PCR molecular detection of Amphidomataceae, with chemical analysis of AZA, and with the establishment and characterization of amphidomatacean strains. Both light microscopy and PCR revealed that Amphidomataceae were widely present in spring plankton communities along the El Rincón area. They were particularly abundant offshore at the shelf front, reaching peak densities of 2.8 × 105 cells L−1, but no AZA were detected in field samples. In total, 31 new strains were determined as Az. dalianense and Az. spinosum, respectively. All Az. dalianense were non-toxigenic and shared the same rRNA sequences. The large majority of the new Az. spinosum strains revealed for the first time the presence of a non-toxigenic ribotype of this species, which is otherwise the most important AZA producer in European waters. One of the new Az. spinosum strains, with a particular slender shape and some other morphological peculiarities, clustered with toxigenic strains of Az. spinosum from Norway and, exceptionally for the species, produced only AZA-2 but not AZA-1. Results indicate a wide diversity within Az. spinosum, both in terms of sequence data and toxin profiles, which also will affect the qualitative and quantitative performance of the specific qPCR assay for this species. Overall, the new data provide a more differentiated perspective of diversity, toxin productivity and occurrence of Amphidomataceae in a poorly explored region of the global ocean.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ARGENTINIAN SHELF
dc.subject
AZADINIUM DALIANENSE
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AZADINIUM SPINOSUM
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AZASPIRACIDS
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DELIMITATION
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GENETIC DISTANCE
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INTRASPECIFIC VARIABILITY
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RIBOTYPE
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SPRING BLOOM
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Oceanografía, Hidrología, Recursos Hídricos
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Ciencias de la Tierra y relacionadas con el Medio Ambiente
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CIENCIAS NATURALES Y EXACTAS
dc.title
High abundance of Amphidomataceae (Dinophyceae) during the 2015 spring bloom of the Argentinean Shelf and a new, non-toxigenic ribotype of Azadinium spinosum
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-03-25T13:40:41Z
dc.journal.volume
84
dc.journal.pagination
244-260
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Tillmann, Urban. Alfred-Wegener-Institut. Helmholtz-Zentrum für Polar und Meeresforschung; Alemania
dc.description.fil
Fil: Gottschling, Marc. Ludwig Maximilians Universitat; Alemania
dc.description.fil
Fil: Krock, Bernd. Alfred-Wegener-Institut. Helmholtz-Zentrum für Polar und Meeresforschung; Alemania
dc.description.fil
Fil: Smith, Kirsty F.. Cawthron Institute; Nueva Zelanda
dc.description.fil
Fil: Guinder, Valeria Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentina
dc.journal.title
Harmful Algae
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.hal.2019.01.008
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S1568988319300149
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