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dc.contributor.author
Gonorazky, Ana Gabriela
dc.contributor.author
Laxalt, Ana Maria
dc.contributor.author
Dekker, H. L.
dc.contributor.author
Rep, M.
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Munnik, T.
dc.contributor.author
Testerink, C.
dc.contributor.author
de la Canal, L
dc.date.available
2017-02-17T14:55:33Z
dc.date.issued
2012-01
dc.identifier.citation
Gonorazky, Ana Gabriela; Laxalt, Ana Maria; Dekker, H. L.; Rep, M.; Munnik, T.; et al.; Phosphatidylinositol 4-phosphate is associated to extracellular lipoproteic fractions and is detected in tomato apoplastic fluids; Wiley; Plant Biology; 14; 1; 1-2012; 41-49
dc.identifier.issn
1435-8603
dc.identifier.uri
http://hdl.handle.net/11336/13122
dc.description.abstract
We have recently detected phosphatidylinositol-4-phosphate (PI4P) in the extracellular medium of tomato cell suspensions. Extracellular PI4P was shown to trigger the activation of defence responses induced by the fungal elicitor xylanase. In this study, by applying a differential centrifugation technique, we found that extracellular PI4P is associated with fractions composed of diverse phospholipids and proteins, which were pelleted from the extracellular medium of tomato cell suspensions grown under basal conditions. Using mass spectrometry, we identified the proteins present in these pelleted fractions. Most of these proteins have previously been characterised as having a role in defence responses. Next, we evaluated whether PI4P could also be detected in an entire plant system. For this, apoplastic fluids of tomato plants grown under basal conditions were analysed using a lipid overlay assay. Interestingly, PI4P could be detected in intercellular fluids obtained from tomato leaflets and xylem sap of tomato plants. By employing electrospray ionisation tandem mass spectrometry (ESI-MS/MS), other phospholipids were also found in intercellular fluids of tomato plants. These had a markedly different profile from the phospholipid pattern identified in entire leaflets. Based on these results, the potential role of extracellular phospholipids in plant intercellular communication is discussed.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Apoplast
dc.subject
Extracellular Medium
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Lipid Signalling
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Lysophospholipid
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Phosphatidic Acid
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Phosphatidylinositol 4-Phosphate
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Phosphoinositide
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Phosphatidylinositol 4-phosphate is associated to extracellular lipoproteic fractions and is detected in tomato apoplastic fluids
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-02-10T18:13:12Z
dc.identifier.eissn
1438-8677
dc.journal.volume
14
dc.journal.number
1
dc.journal.pagination
41-49
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Hoboken
dc.description.fil
Fil: Gonorazky, Ana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
dc.description.fil
Fil: Laxalt, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
dc.description.fil
Fil: Dekker, H. L.. University Of Amsterdam; Países Bajos
dc.description.fil
Fil: Rep, M.. University Of Amsterdam; Países Bajos
dc.description.fil
Fil: Munnik, T.. University Of Amsterdam; Países Bajos
dc.description.fil
Fil: Testerink, C.. University Of Amsterdam; Países Bajos
dc.description.fil
Fil: de la Canal, L. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
dc.journal.title
Plant Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1438-8677.2011.00488.x/abstract
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/j.1438-8677.2011.00488.x
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