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dc.contributor.author Caballero, Veronica Jorgelina
dc.contributor.author Mendieta, Julieta Renee
dc.contributor.author Lombardo, Daniel
dc.contributor.author Saceda, Miguel
dc.contributor.author Ferragut, José Antonio
dc.contributor.author Conde, Ruben Danilo
dc.contributor.author Giudici, Ana Marcela
dc.date.available 2017-02-16T13:55:29Z
dc.date.issued 2014-11
dc.identifier.citation Caballero, Veronica Jorgelina; Mendieta, Julieta Renee; Lombardo, Daniel; Saceda, Miguel; Ferragut, José Antonio; et al.; Liver damage and caspase-dependent apoptosis is related to protein malnutrition in mice: Effect of methionine; Elsevier Gmbh; Acta Histochemica; 117; 1; 11-2014; 126-135
dc.identifier.issn 0065-1281
dc.identifier.uri http://hdl.handle.net/11336/13066
dc.description.abstract This study aimed to determine whether the effects on the mouse liver caused by three periods of feeding a protein-free diet for 5 days followed by a normal complete diet for 5 days (3PFD-CD) are prevented by a constant methionine supply (3PFD + Met-CD). The expressions of carbonic anhydrase III (CAIII), fatty acid synthase (FAS), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and glutathione S-transferase P1 (GSTP1) were assessed by proteomics and reverse transcriptase-polymerase chain reactions. The liver redox status was examined by measuring the activities of superoxide dismutase (SOD) and catalase (CAT), as well as protein carbonylation. Because oxidative stress can result in apoptosis, the activity and content of caspase-3, as well as the x-linked inhibitor of the apoptosis protein (XIAP) and mitochondrial caspase-independent apoptosis inducing factor (AIF) contents were assessed. In addition, the liver histomorphology was examined. Compared to the controls fed a normal complete diet throughout, feeding with 3PFD-CD increased the FAS content, decreased the CAIII content, decreased both the SOD and CAT activities, and increased protein carbonylation. It also activated caspase-3, decreased the XIAP content, decreased the AIF content, increased the number of GSTP1-positive foci and caspase-3-positive cells, and caused fatty livers. Conversely, the changes were lessened to varying degrees in mice fed 3PFD + Met-CD. The present results indicate that a regular Met supply lessens the biochemical changes, damage, and caspase-dependent apoptosis provoked by recurrent dietary amino acid deprivation in the mouse liver.
dc.format application/pdf
dc.language.iso eng
dc.publisher Elsevier Gmbh
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject apoptosis
dc.subject protein depletion
dc.subject liver
dc.subject methionine
dc.subject mice
dc.subject.classification Bioquímica y Biología Molecular
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Liver damage and caspase-dependent apoptosis is related to protein malnutrition in mice: Effect of methionine
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-02-10T18:13:29Z
dc.journal.volume 117
dc.journal.number 1
dc.journal.pagination 126-135
dc.journal.pais Alemania
dc.journal.ciudad Munich
dc.description.fil Fil: Caballero, Veronica Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
dc.description.fil Fil: Mendieta, Julieta Renee. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
dc.description.fil Fil: Lombardo, Daniel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
dc.description.fil Fil: Saceda, Miguel. Universidad de Miguel Hernandez. Centro de Biologia Molecular y Celular; España
dc.description.fil Fil: Ferragut, José Antonio. Universidad de Miguel Hernandez. Centro de Biologia Molecular y Celular; España
dc.description.fil Fil: Conde, Ruben Danilo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
dc.description.fil Fil: Giudici, Ana Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
dc.journal.title Acta Histochemica
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0065128114002888
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.acthis.2014.11.012


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)