Artículo
Liver damage and caspase-dependent apoptosis is related to protein malnutrition in mice: Effect of methionine
Caballero, Veronica Jorgelina
; Mendieta, Julieta Renee; Lombardo, Daniel; Saceda, Miguel; Ferragut, José Antonio; Conde, Ruben Danilo
; Giudici, Ana Marcela
Fecha de publicación:
11/2014
Editorial:
Elsevier Gmbh
Revista:
Acta Histochemica
ISSN:
0065-1281
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
This study aimed to determine whether the effects on the mouse liver caused by three periods of feeding a protein-free diet for 5 days followed by a normal complete diet for 5 days (3PFD-CD) are prevented by a constant methionine supply (3PFD + Met-CD). The expressions of carbonic anhydrase III (CAIII), fatty acid synthase (FAS), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and glutathione S-transferase P1 (GSTP1) were assessed by proteomics and reverse transcriptase-polymerase chain reactions. The liver redox status was examined by measuring the activities of superoxide dismutase (SOD) and catalase (CAT), as well as protein carbonylation. Because oxidative stress can result in apoptosis, the activity and content of caspase-3, as well as the x-linked inhibitor of the apoptosis protein (XIAP) and mitochondrial caspase-independent apoptosis inducing factor (AIF) contents were assessed. In addition, the liver histomorphology was examined. Compared to the controls fed a normal complete diet throughout, feeding with 3PFD-CD increased the FAS content, decreased the CAIII content, decreased both the SOD and CAT activities, and increased protein carbonylation. It also activated caspase-3, decreased the XIAP content, decreased the AIF content, increased the number of GSTP1-positive foci and caspase-3-positive cells, and caused fatty livers. Conversely, the changes were lessened to varying degrees in mice fed 3PFD + Met-CD. The present results indicate that a regular Met supply lessens the biochemical changes, damage, and caspase-dependent apoptosis provoked by recurrent dietary amino acid deprivation in the mouse liver.
Palabras clave:
Apoptosis
,
Protein Depletion
,
Liver
,
Methionine
,
Mice
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Articulos(IIB)
Articulos de INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Articulos de INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Citación
Caballero, Veronica Jorgelina; Mendieta, Julieta Renee; Lombardo, Daniel; Saceda, Miguel; Ferragut, José Antonio; et al.; Liver damage and caspase-dependent apoptosis is related to protein
malnutrition in mice: Effect of methionine; Elsevier Gmbh; Acta Histochemica; 117; 1; 11-2014; 126-135
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