Immobilization of lipase from Candida rugosa on synthesized hydrogel for hydrolysis reaction

Abstract

Novel hydrogels based on N-acryloyl-tris(hydroxymethyl) aminomethane (NAT), 2-aminoethyl methacrylate (AEMA) and N,N-methylenebisacrylamide (BIS) were prepared and applied for immobilization of lipase. Two procedures were previously performed on the hydrogels as follows: (a) modification with epichlorohydrin (ECH) or 1,4-butanediol diglycidyl ether (BDGE) or (b) grafting with AEMA, using ceric ammonium sulfate as an initiator, and subsequent reaction with ECH. In the hydrolysis reactions, high percentages of lipase activity were reached with products whose enzyme immobilization were performed on both BDGE-containing products and grafted matrices, since the enzyme was bound spaced from the matrices and more likely to interact with the substrate. The best derivatives preserved 86.7?89.5% of the activity corresponding to the soluble enzyme.