Artículo
Differential modulation after feeding in different salinities and response to ABA and extracellular Ca++ of APN activity in hepatopancreas of the intertidal euryhaline crab Neohelice granulata
Fecha de publicación:
04/2020
Editorial:
National Research Council Canada-NRC Research Press
Revista:
Canadian Journal of Zoology
ISSN:
0008-4301
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Modulation of aminopeptidase N (APN) activity in the digestive tract by various factors would be important to adjust digestive and absorptive processes under different physiological and (or) environmental conditions. We studied the postprandial responses at different salinities and the effect of abscisic acid (ABA) and extracellular Ca2+ on APN activity in the hepatopancreas (the main site for nutrient digestion and absorption) of the model species Neohelice granulata (Dana, 1851). Enzyme activity was determined at different times (0, 24, 48, and 72 h) after feeding in crabs acclimated either to 35 psu (osmoconformation) or 10 psu (hyper-regulation). APN activity increased around 50% at 24 h after feeding at 35 psu, whereas no changes occurred at 10 psu. Enzyme activity was also assayed in the presence of ABA (1 × 10–4 mol・L–1) or extracellular Ca2+ (1 × 10–4 mol・L–1), showing increments of 60% and 56%, respectively. The results suggest a role of APN in postprandial adjustments and its modulation by different chemical messengers by direct effect on the hepatopancreas. Moreover, to our knowledge, this work is the first to show the effect of ABA on a digestive enzyme in the digestive tract of an animal.
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Articulos(IIB)
Articulos de INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Articulos de INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Articulos(IIMYC)
Articulos de INSTITUTO DE INVESTIGACIONES MARINAS Y COSTERAS
Articulos de INSTITUTO DE INVESTIGACIONES MARINAS Y COSTERAS
Citación
Michiels, Maria Soledad; Daleo, Gustavo Raul; Lopez Mañanes, Alejandra Antonia; Differential modulation after feeding in different salinities and response to ABA and extracellular Ca++ of APN activity in hepatopancreas of the intertidal euryhaline crab Neohelice granulata; National Research Council Canada-NRC Research Press; Canadian Journal of Zoology; 98; 4; 4-2020; 262-268
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