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dc.contributor.author
Borges, Dyego Gonçalves Lino
dc.contributor.author
de Araújo, Marcelo Augusto
dc.contributor.author
Carollo, Carlos Alexandre
dc.contributor.author
Hellmann Carollo, Aline Regina
dc.contributor.author
Lifschitz, Adrian Luis
dc.contributor.author
Conde, Mário Henrique
dc.contributor.author
Green de Freitas, Mariana
dc.contributor.author
dos Santos Freire, Zelina
dc.contributor.author
Francielle Tutija, Juliane
dc.contributor.author
Takemi Muchon Nakatani, Matheus
dc.contributor.author
de Almeida Borges, Fernando
dc.date.available
2021-03-18T14:35:17Z
dc.date.issued
2020-01
dc.identifier.citation
Borges, Dyego Gonçalves Lino; de Araújo, Marcelo Augusto; Carollo, Carlos Alexandre; Hellmann Carollo, Aline Regina; Lifschitz, Adrian Luis; et al.; Combination of quercetin and ivermectin: In vitro and in vivo effects against Haemonchus contortus; Elsevier Science; Acta Tropica; 201; 1-2020; 1-8
dc.identifier.issn
0001-706X
dc.identifier.uri
http://hdl.handle.net/11336/128518
dc.description.abstract
The aim of the present study was to evaluate the in vitro effect of quercetin combined with ivermectin (IVM) on Haemonchus contortus larvae and adults with different resistance profiles and demonstrate the in vivo anthelmintic action of this combination when used in sheep naturally infected. The effect of combination was evaluated based on the analysis of the mean effective concentration (EC50) obtained for larvae using the larval migration inhibition test and for adults using the motility test on females. The tests with larvae and adults were conducted using isolates with different degrees of susceptibility to IVM (sensitive, intermediate and highly resistant). The in vivo effect was evaluated based on the reduction in the egg count (FEC) and reduction in the count of adult helminths recovered after parasitological necropsy. Using the combination of quercetin with IVM, it was observed that in larvae, quercetin did not significantly reduce the EC50 for IVM in the sensitive and highly resistant isolates, but led to a significant reduction in the EC50 for IVM in the intermediate isolate. In adults, quercetin did not significantly reduce the EC50 for IVM in any of the isolates. No significant effect of the combination was found regarding the reduction in FEC or total count of parasites. The results of the in vitro and in vivo tests performed in the present study on quercetin activity underscore the importance of evaluating resistance-reversing agents among different stages of parasite development as well as among isolates with different resistance profiles. The action of quercetin combined with IVM on the motility of H. contortus larvae and adults was influenced by the degree of resistance and development stage of the parasite. The combination was effective only on intermediate resistant larvae. No action of the combination against adults was found. Moreover, this combination, when administered through the intra-abomasal route, was not effective at reducing the FEC and parasite load of naturally infected sheep.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ANTHELMINTIC RESISTANCE
dc.subject
FLAVONOIDS
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MODULATION
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PARASITES
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RUMINANTS
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Ciencias Veterinarias
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Ciencias Veterinarias
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CIENCIAS AGRÍCOLAS
dc.title
Combination of quercetin and ivermectin: In vitro and in vivo effects against Haemonchus contortus
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-03-15T14:33:12Z
dc.journal.volume
201
dc.journal.pagination
1-8
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Borges, Dyego Gonçalves Lino. Universidade Federal do Mato Grosso do Sul; Brasil
dc.description.fil
Fil: de Araújo, Marcelo Augusto. Universidade Federal do Mato Grosso do Sul; Brasil
dc.description.fil
Fil: Carollo, Carlos Alexandre. Universidade Federal do Mato Grosso do Sul; Brasil
dc.description.fil
Fil: Hellmann Carollo, Aline Regina. Universidade Federal do Mato Grosso do Sul; Brasil
dc.description.fil
Fil: Lifschitz, Adrian Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
dc.description.fil
Fil: Conde, Mário Henrique. Universidade Federal do Mato Grosso do Sul; Brasil
dc.description.fil
Fil: Green de Freitas, Mariana. Universidade Federal do Mato Grosso do Sul; Brasil
dc.description.fil
Fil: dos Santos Freire, Zelina. Universidade Federal do Mato Grosso do Sul; Brasil
dc.description.fil
Fil: Francielle Tutija, Juliane. Universidade Federal do Mato Grosso do Sul; Brasil
dc.description.fil
Fil: Takemi Muchon Nakatani, Matheus. Universidade Federal do Mato Grosso do Sul; Brasil
dc.description.fil
Fil: de Almeida Borges, Fernando. Universidade Federal do Mato Grosso do Sul; Brasil
dc.journal.title
Acta Tropica
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.actatropica.2019.105213
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0001706X19306023?via%3Dihub
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