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dc.contributor.author
Formenti, Fabio
dc.contributor.author
La Marca, Giulia
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Perandin, Francesca
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Pajola, Barbara
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Romano, Miryam de Los Angeles
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Santucci, Beatrice
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Silva, Ronaldo
dc.contributor.author
Giorli, Giovanni
dc.contributor.author
Bisoffi, Zeno
dc.contributor.author
Buonfrate, Dora
dc.date.available
2020-11-11T20:12:19Z
dc.date.issued
2019-02
dc.identifier.citation
Formenti, Fabio; La Marca, Giulia; Perandin, Francesca; Pajola, Barbara; Romano, Miryam de Los Angeles; et al.; A diagnostic study comparing conventional and real-time PCR for Strongyloides stercoralis on urine and on faecal samples; Elsevier Science; Acta Tropica; 190; 2-2019; 284-287
dc.identifier.issn
0001-706X
dc.identifier.uri
http://hdl.handle.net/11336/118219
dc.description.abstract
Strongyloides stercoralis is a soil-transmitted helminth with a wide distribution in tropical and subtropical areas. The diagnosis of S. stercoralisinfection can be challenging, due to the low sensitivity of microscopic examination of stool samples and coproculture. In the last decade, different in-house molecular biology techniques for S. stercoralis have been implemented. They demonstrated good accuracy, although sensitivity does not seem sufficiently high yet. Recently, a novel PCR technique has been evaluated for the detection of S. stercoralis DNA in urine. Aim of this work was to compare the sensitivity of the real-time PCR (qPCR) on feces routinely used at the Centre for Tropical Disease (CTD) of Negrar, Verona, Italy, with that of the novel based PCR on urine. As secondary objective, we evaluated a Urine Conditioning Buffer ® (Zymoresearch) with the aim of improving nucleic acid stability in urine during sample storage/transport at ambient temperatures. Patients attending the CTD and resulting positive at routine screening with serology for S. stercoralis were invited, previous written consent, to supply stool and urine samples for molecular biology. A convenience sample of 30 patients was included. The sensitivity of qPCR on feces resulted 63%, and that of based PCR on urine was 17%. In all the samples treated with the Urine Conditioning Buffer ® there was no detectable DNA. In conclusion, the sensitivity of the novel technique resulted low, and needs further implementation before being considered as a valid alternative to the validated method.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
DIAGNOSIS
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DIAGNOSTIC TEST
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DNA
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FECAL SPECIMEN
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STRONGYLOIDES STERCORALIS
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URINE SPECIMEN
dc.subject.classification
Enfermedades Infecciosas
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Ciencias de la Salud
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
A diagnostic study comparing conventional and real-time PCR for Strongyloides stercoralis on urine and on faecal samples
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-05-19T19:48:40Z
dc.journal.volume
190
dc.journal.pagination
284-287
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Formenti, Fabio. Sacro Cuore Don Calabria General Hospital; Italia
dc.description.fil
Fil: La Marca, Giulia. Sacro Cuore Don Calabria General Hospital; Italia
dc.description.fil
Fil: Perandin, Francesca. Sacro Cuore Don Calabria General Hospital; Italia
dc.description.fil
Fil: Pajola, Barbara. Sacro Cuore Don Calabria General Hospital; Italia
dc.description.fil
Fil: Romano, Miryam de Los Angeles. University Johns Hopkins; Estados Unidos. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Laboratorio de Patología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina
dc.description.fil
Fil: Santucci, Beatrice. Sacro Cuore Don Calabria General Hospital; Italia
dc.description.fil
Fil: Silva, Ronaldo. Sacro Cuore Don Calabria General Hospital; Italia
dc.description.fil
Fil: Giorli, Giovanni. Sacro Cuore Don Calabria General Hospital; Italia
dc.description.fil
Fil: Bisoffi, Zeno. Universita di Verona; Italia. Sacro Cuore Don Calabria General Hospital; Italia
dc.description.fil
Fil: Buonfrate, Dora. Sacro Cuore Don Calabria General Hospital; Italia
dc.journal.title
Acta Tropica
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.actatropica.2018.12.001
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0001706X18307605
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