Artículo
A diagnostic study comparing conventional and real-time PCR for Strongyloides stercoralis on urine and on faecal samples
Formenti, Fabio; La Marca, Giulia; Perandin, Francesca; Pajola, Barbara; Romano, Miryam de Los Angeles
; Santucci, Beatrice; Silva, Ronaldo; Giorli, Giovanni; Bisoffi, Zeno; Buonfrate, Dora
Fecha de publicación:
02/2019
Editorial:
Elsevier Science
Revista:
Acta Tropica
ISSN:
0001-706X
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Strongyloides stercoralis is a soil-transmitted helminth with a wide distribution in tropical and subtropical areas. The diagnosis of S. stercoralisinfection can be challenging, due to the low sensitivity of microscopic examination of stool samples and coproculture. In the last decade, different in-house molecular biology techniques for S. stercoralis have been implemented. They demonstrated good accuracy, although sensitivity does not seem sufficiently high yet. Recently, a novel PCR technique has been evaluated for the detection of S. stercoralis DNA in urine. Aim of this work was to compare the sensitivity of the real-time PCR (qPCR) on feces routinely used at the Centre for Tropical Disease (CTD) of Negrar, Verona, Italy, with that of the novel based PCR on urine. As secondary objective, we evaluated a Urine Conditioning Buffer ® (Zymoresearch) with the aim of improving nucleic acid stability in urine during sample storage/transport at ambient temperatures. Patients attending the CTD and resulting positive at routine screening with serology for S. stercoralis were invited, previous written consent, to supply stool and urine samples for molecular biology. A convenience sample of 30 patients was included. The sensitivity of qPCR on feces resulted 63%, and that of based PCR on urine was 17%. In all the samples treated with the Urine Conditioning Buffer ® there was no detectable DNA. In conclusion, the sensitivity of the novel technique resulted low, and needs further implementation before being considered as a valid alternative to the validated method.
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Articulos(IPE)
Articulos de INST.DE PATOLOGIA EXPERIMENTAL
Articulos de INST.DE PATOLOGIA EXPERIMENTAL
Citación
Formenti, Fabio; La Marca, Giulia; Perandin, Francesca; Pajola, Barbara; Romano, Miryam de Los Angeles; et al.; A diagnostic study comparing conventional and real-time PCR for Strongyloides stercoralis on urine and on faecal samples; Elsevier Science; Acta Tropica; 190; 2-2019; 284-287
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