Mostrar el registro sencillo del ítem
dc.contributor.author
Rodríguez Carrio, Javier
dc.contributor.author
Carrillo López, Natalia
dc.contributor.author
Ulloa, Catalina
dc.contributor.author
Seijo, Mariana
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Rodríguez García, Minerva
dc.contributor.author
Rodríguez Suárez, Carmen
dc.contributor.author
Díaz-Corte, Carmen
dc.contributor.author
Cannata Andía, Jorge B.
dc.contributor.author
Suárez, Ana
dc.contributor.author
Dusso, Adriana
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.date.available
2020-10-20T15:13:40Z
dc.date.issued
2019-09-13
dc.identifier.citation
Rodríguez Carrio, Javier; Carrillo López, Natalia; Ulloa, Catalina; Seijo, Mariana; Rodríguez García, Minerva; et al.; A subset of low density granulocytes is associated with vascular calcification in chronic kidney disease patients; Nature Publishing Group; Scientific Reports; 9; 1; 13-9-2019; 1-11
dc.identifier.issn
2045-2322
dc.identifier.uri
http://hdl.handle.net/11336/116165
dc.description.abstract
Inflammation is central to chronic kidney disease (CKD) pathogenesis and vascular outcomes, but the exact players remain unidentified. Since low density granulocytes (LDGs) are emerging mediators in inflammatory conditions, we aimed to evaluate whether LDGs may be altered in CKD and related to clinical outcomes as biomarkers. To his end, LDGs subsets were measured in peripheral blood by flow cytometry and confocal microscopy in 33 CKD patients undergoing peritoneal dialysis and 15 healthy controls (HC). Analyses were replicated in an additional cohort. DEF3 (marker of early granulopoiesis) gene expression on PBMCs was quantified by qPCR. Total CD15+ LDGs and both CD14lowCD16+ and CD14−CD16− subsets were expanded in CKD. The relative frequency of the CD14−CD16− subpopulation was higher among the CD15+ pool in CKD. This alteration was stable over-time. The increased CD14−CD16−CD15+ paralleled Kauppila scores and DEF3 expression, whereas no association was found with CD14lowCD16+ CD15+. Both subsets differed in their CD11b, CD10, CD35, CD31, CD62L, IFNAR1 and CD68 expression, FSC/SSC features and nuclear morphology, pointing to different origins and maturation status. In conclusion, LDGs were expanded in CKD showing a skewed distribution towards a CD14−CD16−CD15+ enrichment, in association with vascular calcification. DEF3 expression in PBMC can be a marker of LDG expansion.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Nature Publishing Group
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
diagnostic markers
dc.subject
traslational research
dc.subject
kidney disease
dc.subject.classification
Otras Ciencias de la Salud
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
Ciencias de la Salud
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.title
A subset of low density granulocytes is associated with vascular calcification in chronic kidney disease patients
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-07-21T20:19:10Z
dc.journal.volume
9
dc.journal.number
1
dc.journal.pagination
1-11
dc.journal.pais
Reino Unido
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.journal.ciudad
Londres
dc.description.fil
Fil: Rodríguez Carrio, Javier. Hospital Universitario Central de Asturias. Instituto de Investigación Sanitaria del Principado de Asturias (ISPA). Bone and Mineral Research Unit; España. Universidad de Oviedo; España
dc.description.fil
Fil: Carrillo López, Natalia. Hospital Universitario Central de Asturias; España
dc.description.fil
Fil: Ulloa, Catalina. Hospital Universitario Central de Asturias; España
dc.description.fil
Fil: Seijo, Mariana. Hospital Universitario Central de Asturias; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
dc.description.fil
Fil: Rodríguez García, Minerva. Hospital Universitario Central de Asturias; España
dc.description.fil
Fil: Rodríguez Suárez, Carmen. Hospital Universitario Central de Asturias; España
dc.description.fil
Fil: Díaz-Corte, Carmen. Hospital Universitario Central de Asturias; España
dc.description.fil
Fil: Cannata Andía, Jorge B.. Universidad de Oviedo; España. Hospital Universitario Central de Asturias; España
dc.description.fil
Fil: Suárez, Ana. Universidad de Oviedo; España
dc.description.fil
Fil: Dusso, Adriana. Hospital Universitario Central de Asturias; España
dc.journal.title
Scientific Reports
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/s41598-019-49429-x
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1038/s41598-019-49429-x
Archivos asociados