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dc.contributor.author
Alarcón, Ramiro
dc.contributor.author
Rivera, Oscar Edgardo
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Ingaramo, Paola Inés
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Tschopp, María Virginia
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Dioguardi, Gisela Haydeé
dc.contributor.author
Milesi, Maria Mercedes
dc.contributor.author
Muñoz de Toro, Monica Milagros
dc.contributor.author
Luque, Enrique Hugo
dc.date.available
2020-07-26T15:09:14Z
dc.date.issued
2020-10
dc.identifier.citation
Alarcón, Ramiro; Rivera, Oscar Edgardo; Ingaramo, Paola Inés; Tschopp, María Virginia; Dioguardi, Gisela Haydeé; et al.; Neonatal exposure to a glyphosate-based herbicide alters the uterine differentiation of prepubertal ewe lambs; Elsevier; Environmental Pollution; 265; 10-2020
dc.identifier.issn
0269-7491
dc.identifier.uri
http://hdl.handle.net/11336/110284
dc.description.abstract
The exposure to endocrine-disrupting compounds (EDCs), such as glyphosate-based herbicides (GBHs), during early life might alter female fertility. The aim of the present study was to evaluate the effects of neonatal exposure to a GBH on sheep uterine development. To achieve this, Friesian ewe lambs were exposed to GBH (2 mg/kg of body weight/day; n = 12) or vehicle (controls; n = 10) through s.c. injections, from postnatal day (PND) 1 to PND14; on PND45, the uteri were obtained to evaluate histomorphological and molecular parameters. Morphological parameters were determined by picrosirius-hematoxylin staining. Protein expression of Ki67 (as a cell proliferation marker), p27, and molecules involved in uterine organogenetic differentiation was measured by immunohistochemistry. We also determined the mRNA expression of the IGF molecular pathway by RT-PCR. Although histomorphology was not modified, the uteri of GBH-exposed ewe lambs showed lower cell proliferation, together with higher p27 protein expression. In addition, the uteri of GBH-exposed ewe lambs showed increased gene expression of insulin-like growth factor binding protein 3 (IGFBP-3), decreased expression of ERα in the luminal (LE) and glandular (GE) epithelia and in the subepithelial stroma (SS), and lower PR expression in the LE but higher in the GE and SS. In addition, GBH treatment decreased the uterine expression of Wnt5a in the GE, of Wnt7a in the SS, of β-catenin in the LE and GE, of Hoxa10 in the SS, and of Foxa2 in the GE as compared with controls. In conclusion, neonatal exposure to GBH decreased cell proliferation and altered the expression of molecules that control proliferation and development in the uterus. All these changes might have adverse consequences on uterine differentiation and functionality, affecting the female reproductive health of sheep. GBH may be responsible for uterine subfertility, acting as an EDC.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ENVIRONMENTAL HEALTH
dc.subject
UTERINE DEVELOPMENT
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EWE LAMBS
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SHEEP
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ANIMAL PRODUCTION
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FEMALE FERTILITY
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Salud Pública y Medioambiental
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Ciencias de la Salud
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Neonatal exposure to a glyphosate-based herbicide alters the uterine differentiation of prepubertal ewe lambs
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-07-20T16:18:21Z
dc.journal.volume
265
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Alarcón, Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
dc.description.fil
Fil: Rivera, Oscar Edgardo. Universidad Nacional de Lomas de Zamora. Facultad de Cs.agrarias. Instituto de Investigacion En Produccion Agropecuaria Ambiente y Salud. - Comision de Investigaciones Cientificas de la Provincia de Buenos Aires. Instituto de Investigacion En Produccion Agropecuaria Ambiente y Salud.; Argentina
dc.description.fil
Fil: Ingaramo, Paola Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
dc.description.fil
Fil: Tschopp, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
dc.description.fil
Fil: Dioguardi, Gisela Haydeé. Universidad Nacional de Lomas de Zamora. Facultad de Cs.agrarias. Instituto de Investigacion En Produccion Agropecuaria Ambiente y Salud. - Comision de Investigaciones Cientificas de la Provincia de Buenos Aires. Instituto de Investigacion En Produccion Agropecuaria Ambiente y Salud.; Argentina
dc.description.fil
Fil: Milesi, Maria Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
dc.description.fil
Fil: Muñoz de Toro, Monica Milagros. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
dc.description.fil
Fil: Luque, Enrique Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
dc.journal.title
Environmental Pollution
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0269749120312458
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.envpol.2020.114874
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