Artículo
Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942)
Vidal, Virginia; Ortiz, Juana; Diaz, Julia Inés
; Zafrilla, Basilio; Bonete, Maria José; Ruíz de Ybañez, Maria Rocío; Palacios, Maria José; Benzal, Jesús; Valera, Francisco; de la Cruz, Carlos; Motas, Miguel; Bautista, Vanesa; Machordom, Annie; Barbosa, Andrés
Fecha de publicación:
03/2016
Editorial:
Cambridge University Press
Revista:
Journal Of Helminthology
ISSN:
0022-149X
e-ISSN:
1475-2697
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Stegophorus macronectes (Johnston & Mawson, 1942) is a gastrointestinal parasite found in Antarctic seabirds. The original description of the species, which was based only on females, is poor and fragmented with some unclear diagnostic characters. This study provides new morphometric and molecular data on this previously poorly described parasite. Nuclear rDNA sequences (18S, 5.8S, 28S and internal transcribed spacer (ITS) regions) were isolated from S. macronectes specimens collected from the chinstrap penguin Pygoscelis antarctica Forster on Deception Island, Antarctica. Using 18S rDNA sequences, phylogenetic analyses (maximum likelihood, maximum parsimony and Bayesian inference) of the order Spirurida were performed to determine the phylogenetic location of this species. Primer pairs of the ITS regions were designed for genus-level identification of specimens, regardless of their cycle, as an alternative to coprological methods. The utility of this molecular method for identification of morphologically altered specimens is also discussed.
Palabras clave:
Stegophorus Macronectes
,
Pygoscelis Antarctica
,
Penguins
,
Antarctica
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CEPAVE)
Articulos de CENTRO DE EST.PARASITOL.Y DE VECTORES (I)
Articulos de CENTRO DE EST.PARASITOL.Y DE VECTORES (I)
Citación
Vidal, Virginia; Ortiz, Juana; Diaz, Julia Inés; Zafrilla, Basilio; Bonete, Maria José; et al.; Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942); Cambridge University Press; Journal Of Helminthology; 90; 2; 3-2016; 214-222
Compartir
Altmétricas