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dc.contributor.author
Curatti, Leonardo
dc.contributor.author
Rubio, Luis M.
dc.date.available
2017-01-03T15:25:38Z
dc.date.issued
2014-08
dc.identifier.citation
Curatti, Leonardo; Rubio, Luis M.; Challenges to develop nitrogen-fixing cereals by direct nif-gene transfer; Elsevier Ireland; Plant Science; 225; 8-2014; 130–137
dc.identifier.issn
0168-9452
dc.identifier.uri
http://hdl.handle.net/11336/10744
dc.description.abstract
Some regions of the developing world suffer low cereal production yields due to low fertilizer inputs, among other factors. Biological N2 fixation, catalyzed by the prokaryotic enzyme nitrogenase, is an alternative to the use of synthetic N fertilizers. The molybdenum nitrogenase is an O2-labile metalloenzyme composed of the NifDK and NifH proteins, which biosyntheses require a number of nif gene products. A challenging strategy to increase cereal crop productivity in a scenario of low N fertilization is the direct transfer of nif genes into cereals. The sensitivity of nitrogenase to O2 and the apparent complexity of nitrogenase biosynthesis are the main barriers identified so far. Expression of active NifH requires the products of nifM, nifH, and possibly nifU and nifS, whereas active NifDK requires the products of nifH, nifD, nifK, nifB, nifE, nifN, and possibly nifU, nifS, nifQ, nifV, nafY, nifW and nifZ. Plastids and mitochondria are potential subcellular locations for nitrogenase. Both could provide the ATP and electrons required for nitrogenase to function but they differ in their internal O2 levels and their ability to incorporate ammonium into amino acids.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Ireland
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Cereales
dc.subject
Nitrogen Fixation
dc.subject
Genetic Engineering
dc.subject
Fertilizers
dc.subject
Food Security
dc.subject
Nitrogenase
dc.subject
Nif
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Challenges to develop nitrogen-fixing cereals by direct nif-gene transfer
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-01-02T18:33:03Z
dc.journal.volume
225
dc.journal.pagination
130–137
dc.journal.pais
Irlanda
dc.description.fil
Fil: Curatti, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones En Biodiversidad y Biotecnología; Argentina. Fundación para Investigaciones Biológicas Aplicadas; España
dc.description.fil
Fil: Rubio, Luis M.. Universidad Politecnica de Madrid; España
dc.journal.title
Plant Science
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.plantsci.2014.06.003
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S016894521400137X
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