Preliminary cross-genera transferability of SSRs among threatened South American Cupressaceae

Abstract

Development of specific microsatellite primers is a relatively expensive and time-consuming process, so transferring of already developed markers from one species to others is desirable, especially for non-model organisms with large genome structure such as conifers. In austral South America the three Cupressaceae are monotypic genera of high conservation and evolutionary relevance. Although, they had beenthe focus of numerous population genetic studies the lack of highly polymorphic markers as microsatellites for all species, has limited the possibility of evolutionary studies and the design of appropriate conservation plans. Thus the aim of this study is to transfer and test microsatellites primers early designed for Austrocedrus to Fitzroya and Pilgerodendron. Transferability of nine microsatellite primers and their utility were tested by comparing genetic parameters of 20 individuals of each species in six populations where the three species occur. Four markers were simultaneously transferred with success, representing the first study in conifers to achieve cross-genera transferability. This small set of primers yielded high levels of polymorphism for the three studied species and show unique alleles that separate pure taxa. These highly polymorphic markers provide relevant tools to study the evolutionary history of South American Cupressaceae and to generate information that could help to guide conservation efforts.