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dc.contributor.author
Castellaro, Andrés Marcos
dc.contributor.author
Tonda, Alfredo
dc.contributor.author
Cejas, Hugo H.
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Ferreyra, Héctor
dc.contributor.author
Caputto, Beatriz Leonor
dc.contributor.author
Pucci, Oscar A.
dc.contributor.author
Gil, German Alejandro
dc.date.available
2016-12-29T18:57:13Z
dc.date.issued
2015-10-22
dc.identifier.citation
Castellaro, Andrés Marcos; Tonda, Alfredo; Cejas, Hugo H.; Ferreyra, Héctor; Caputto, Beatriz Leonor; et al.; Oxalate induces breast cancer; Biomed Central; Bmc Cancer; 15; 22-10-2015; 761-774
dc.identifier.issn
1471-2407
dc.identifier.uri
http://hdl.handle.net/11336/10634
dc.description.abstract
Background: Microcalcifications can be the early and only presenting sign of breast cancer. One shared characteristic of breast cancer is the appearance of mammographic mammary microcalcifications that can routinely be used to detect breast cancer in its initial stages, which is of key importance due to the possibility that early detection allows the application of more conservative therapies for a better patient outcome. The mechanism by which mammary microcalcifications are formed is still largely unknown but breast cancers presenting microcalcifications are more often associated with a poorer prognosis. Methods: We combined Capillary Electrochromatography, histology, and gene expression (qRT-PCR) to analyze patient-matched normal breast tissue vs. breast tumor. Potential carcinogenicity of oxalate was tested by its inoculation into mice. All data were subjected to statistical analysis. Results: To study the biological significance of oxalates within the breast tumor microenvironment, we measured oxalate concentration in both human breast tumor tissues and adjoining non-pathological breast tissues. We found that all tested breast tumor tissues contain a higher concentration of oxalates than their counterpart nonpathological breast tissue. Moreover, it was established that oxalate induces proliferation of breast cells and stimulates the expression of a pro-tumorigenic gene c-fos. Furthermore, oxalate generates highly malignant and undifferentiated tumors when it was injected into the mammary fatpad in female mice, but not when injected into their back, indicating that oxalate does not induce cancer formation in all types of tissues. Moreover, neither human kidney-epithelial cells nor mouse fibroblast cells proliferate when are treated with oxalate. Conclusions: We found that the chronic exposure of breast epithelial cells to oxalate promotes the transformation of breast cells from normal to tumor cells, inducing the expression of a proto-oncogen as c-fos and proliferation in breast cancer cells. Furthermore, oxalate has a carcinogenic effect when injected into the mammary fatpad in mice, generating highly malignant and undifferentiated tumors with the characteristics of fibrosarcomas of the breast. As oxalates seem to promote these differences, it is expected that a significant reduction in the incidence of breast cancer tumors could be reached if it were possible to control oxalate production or its carcinogenic activity.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Biomed Central
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
Microcalcifications
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Oxalate
dc.subject
Calcium Oxalate
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Breast Cancer Induction
dc.subject.classification
Bioquímica y Biología Molecular
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Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Oxalate induces breast cancer
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-12-28T17:55:11Z
dc.journal.volume
15
dc.journal.pagination
761-774
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Castellaro, Andrés Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Quimica Biológica; Argentina
dc.description.fil
Fil: Tonda, Alfredo. Universidad Nacional de Cordoba. Facultad de Medicina. Hospital Nacional de Clinicas; Argentina
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Fil: Cejas, Hugo H.. Universidad Nacional de Cordoba. Facultad de Medicina. Hospital Nacional de Clinicas; Argentina
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Fil: Ferreyra, Héctor. Universidad Nacional de Cordoba. Facultad de Medicina. Hospital Nacional de Clinicas; Argentina
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Fil: Caputto, Beatriz Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Quimica Biológica; Argentina
dc.description.fil
Fil: Pucci, Oscar A.. Universidad Nacional de Cordoba. Facultad de Medicina. Hospital Nacional de Clinicas; Argentina
dc.description.fil
Fil: Gil, German Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Química Biológica de Córdoba (p); Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Quimica Biológica; Argentina
dc.journal.title
Bmc Cancer
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1186/s12885-015-1747-2
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.biomedcentral.com/1471-2407/15/761
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618885/


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