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dc.contributor.author Castellaro, Andrés Marcos
dc.contributor.author Tonda, Alfredo
dc.contributor.author Cejas, Hugo H.
dc.contributor.author Ferreyra, Héctor
dc.contributor.author Caputto, Beatriz Leonor
dc.contributor.author Pucci, Oscar A.
dc.contributor.author Gil, German Alejandro
dc.date.available 2016-12-29T18:57:13Z
dc.date.issued 2015-10-22
dc.identifier.citation Castellaro, Andrés Marcos; Tonda, Alfredo; Cejas, Hugo H.; Ferreyra, Héctor; Caputto, Beatriz Leonor; et al.; Oxalate induces breast cancer; Biomed Central; Bmc Cancer; 15; 22-10-2015; 761-774
dc.identifier.issn 1471-2407
dc.identifier.uri http://hdl.handle.net/11336/10634
dc.description.abstract Background: Microcalcifications can be the early and only presenting sign of breast cancer. One shared characteristic of breast cancer is the appearance of mammographic mammary microcalcifications that can routinely be used to detect breast cancer in its initial stages, which is of key importance due to the possibility that early detection allows the application of more conservative therapies for a better patient outcome. The mechanism by which mammary microcalcifications are formed is still largely unknown but breast cancers presenting microcalcifications are more often associated with a poorer prognosis. Methods: We combined Capillary Electrochromatography, histology, and gene expression (qRT-PCR) to analyze patient-matched normal breast tissue vs. breast tumor. Potential carcinogenicity of oxalate was tested by its inoculation into mice. All data were subjected to statistical analysis. Results: To study the biological significance of oxalates within the breast tumor microenvironment, we measured oxalate concentration in both human breast tumor tissues and adjoining non-pathological breast tissues. We found that all tested breast tumor tissues contain a higher concentration of oxalates than their counterpart nonpathological breast tissue. Moreover, it was established that oxalate induces proliferation of breast cells and stimulates the expression of a pro-tumorigenic gene c-fos. Furthermore, oxalate generates highly malignant and undifferentiated tumors when it was injected into the mammary fatpad in female mice, but not when injected into their back, indicating that oxalate does not induce cancer formation in all types of tissues. Moreover, neither human kidney-epithelial cells nor mouse fibroblast cells proliferate when are treated with oxalate. Conclusions: We found that the chronic exposure of breast epithelial cells to oxalate promotes the transformation of breast cells from normal to tumor cells, inducing the expression of a proto-oncogen as c-fos and proliferation in breast cancer cells. Furthermore, oxalate has a carcinogenic effect when injected into the mammary fatpad in mice, generating highly malignant and undifferentiated tumors with the characteristics of fibrosarcomas of the breast. As oxalates seem to promote these differences, it is expected that a significant reduction in the incidence of breast cancer tumors could be reached if it were possible to control oxalate production or its carcinogenic activity.
dc.format application/pdf
dc.language.iso eng
dc.publisher Biomed Central
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by/2.5/ar/
dc.subject Microcalcifications
dc.subject Oxalate
dc.subject Calcium Oxalate
dc.subject Breast Cancer Induction
dc.subject.classification Bioquímica y Biología Molecular
dc.subject.classification Medicina Básica
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.title Oxalate induces breast cancer
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2016-12-28T17:55:11Z
dc.journal.volume 15
dc.journal.pagination 761-774
dc.journal.pais Reino Unido
dc.journal.ciudad Londres
dc.description.fil Fil: Castellaro, Andrés Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Quimica Biológica; Argentina
dc.description.fil Fil: Tonda, Alfredo. Universidad Nacional de Cordoba. Facultad de Medicina. Hospital Nacional de Clinicas; Argentina
dc.description.fil Fil: Cejas, Hugo H.. Universidad Nacional de Cordoba. Facultad de Medicina. Hospital Nacional de Clinicas; Argentina
dc.description.fil Fil: Ferreyra, Héctor. Universidad Nacional de Cordoba. Facultad de Medicina. Hospital Nacional de Clinicas; Argentina
dc.description.fil Fil: Caputto, Beatriz Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Quimica Biológica; Argentina
dc.description.fil Fil: Pucci, Oscar A.. Universidad Nacional de Cordoba. Facultad de Medicina. Hospital Nacional de Clinicas; Argentina
dc.description.fil Fil: Gil, German Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Química Biológica de Córdoba (p); Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Quimica Biológica; Argentina
dc.journal.title Bmc Cancer
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1186/s12885-015-1747-2
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.biomedcentral.com/1471-2407/15/761
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618885/


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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution 2.5 Unported (CC BY 2.5)