Mostrar el registro sencillo del ítem
dc.contributor.author
de Sousa, Marylane
dc.contributor.author
Melo, Vânia M. M.
dc.contributor.author
Hissa, Denise C.
dc.contributor.author
Manzo, Ricardo Martín
dc.contributor.author
Mammarella, Enrique José
dc.contributor.author
Antunes, André Saraiva Leão Marcelo
dc.contributor.author
García, José L.
dc.contributor.author
Pessela, Benevides C.
dc.contributor.author
Gonçalves, Luciana R. B.
dc.date.available
2020-05-19T22:55:19Z
dc.date.issued
2019-04
dc.identifier.citation
de Sousa, Marylane; Melo, Vânia M. M.; Hissa, Denise C.; Manzo, Ricardo Martín; Mammarella, Enrique José; et al.; One-Step Immobilization and Stabilization of a Recombinant Enterococcus faecium DBFIQ E36 l-Arabinose Isomerase for d-Tagatose Synthesis; Humana Press; Applied Biochemistry And Biotechnology; 188; 4-2019; 310-325
dc.identifier.issn
0273-2289
dc.identifier.uri
http://hdl.handle.net/11336/105528
dc.description.abstract
A recombinant l-arabinose isomerase from Enterococcus faecium DBFIQ E36 was immobilized onto multifunctional epoxide supports by chemical adsorption and onto a chelate-activated support via polyhistidine-tag, located on the N-terminal (N-His-L-AI) or on the C-terminal (C-His-L-AI) sequence, followed by covalent bonding between the enzyme and the support. The results were compared to reversible L-AI immobilization by adsorption onto charged agarose supports with improved stability. All the derivatives presented immobilization yields of above 75%. The ionic interaction established between agarose gels containing monoaminoethyl-N-aminoethyl structures (MANAE) and the enzyme was the most suitable strategy for L-AI immobilization in comparison to the chelate-activated agarose. In addition, the immobilized biocatalysts by ionic interaction in MANAE showed to be the most stable, retaining up to 100% of enzyme activity for 60 min at 60 °C and with Km values of 28 and 218 mM for MANAE-N-His-L-AI and MANAE-C-His-L-AI, respectively.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Humana Press
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CHELATE-AGAROSE
dc.subject
D-TAGATOSE
dc.subject
ENTEROCOCCUS FAECIUM
dc.subject
ENZYME ACTIVITY
dc.subject
IMMOBILIZATION
dc.subject
L-ARABINOSE ISOMERASE
dc.subject.classification
Bioprocesamiento Tecnológico, Biocatálisis, Fermentación
dc.subject.classification
Biotecnología Industrial
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
dc.title
One-Step Immobilization and Stabilization of a Recombinant Enterococcus faecium DBFIQ E36 l-Arabinose Isomerase for d-Tagatose Synthesis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-05-19T19:49:24Z
dc.journal.volume
188
dc.journal.pagination
310-325
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: de Sousa, Marylane. Universidade Estadual do Ceará; Brasil
dc.description.fil
Fil: Melo, Vânia M. M.. Universidade Estadual do Ceará; Brasil
dc.description.fil
Fil: Hissa, Denise C.. Universidade Estadual do Ceará; Brasil
dc.description.fil
Fil: Manzo, Ricardo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
dc.description.fil
Fil: Mammarella, Enrique José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
dc.description.fil
Fil: Antunes, André Saraiva Leão Marcelo. King's College London;
dc.description.fil
Fil: García, José L.. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España
dc.description.fil
Fil: Pessela, Benevides C.. Polytechnic Institute Of Technology And Sciences; Angola. Csic-uam - Instituto de Investigacion En Ciencias de la Alimentacion (cial);
dc.description.fil
Fil: Gonçalves, Luciana R. B.. Universidade Estadual do Ceará; Brasil
dc.journal.title
Applied Biochemistry And Biotechnology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s12010-018-2905-1
Archivos asociados