Artículo
On-Line Photoinduced Fluorescence for Carbamazepine Determination after Multivariate Optimization
Henestrosa, Cecilia Mariel; Peralta, Cecilia Mariana
; Aragón, Leslie Mary; Fernandez, Liliana Patricia
; Acosta, Maria Gimena
Fecha de publicación:
11/2019
Editorial:
Scientific Research
Revista:
American Journal of Analytical Chemistry
ISSN:
2156-8278
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
This work presents a simple and sensitive flow injection fluorimetric methodfor the determination of carbamazepine (CBZ) in pharmaceutical samples. Itis based on the fluorescence signal measurement of the product generated byphotoinduced (λex = 251 nm; λem = 434 nm) using a flow injection manifoldcontaining an on-line homemade photoreactor. In order to optimize the experimental setup, the variable conditions were studied using a multivariateoptimization method, thus finding the set of optimum parameters accordingto the evaluated responses (sensitivity and sampling frequency) and minimizing the number of experiments performed. Under optimal experimentalconditions, linear relationship with good correlation coefficient (0.9956) wasfound between the fluorescence intensity and CBZ concentration in the rangeof 0.13 - 40 µg∙mL−1. The limit of detection was 0.04 µg∙mL−1. The precision ofthe method was satisfactory; the values of relative standard deviations werebetter than 4.36%. The proposed method was validated and successfully applied to the determination of CBZ in pharmaceuticals with good accuracy;being the recovery values from 89.45% - 112.20%.
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Articulos(INQUISAL)
Articulos de INST. DE QUIMICA DE SAN LUIS
Articulos de INST. DE QUIMICA DE SAN LUIS
Citación
Henestrosa, Cecilia Mariel; Peralta, Cecilia Mariana; Aragón, Leslie Mary; Fernandez, Liliana Patricia; Acosta, Maria Gimena; On-Line Photoinduced Fluorescence for Carbamazepine Determination after Multivariate Optimization; Scientific Research; American Journal of Analytical Chemistry; 10; 11; 11-2019; 513-527
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