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dc.contributor.author
Borgonovo, Janina Edith
dc.contributor.author
Troncoso, Mariana Elizabeth
dc.contributor.author
Lucas, José J.
dc.contributor.author
Sosa Escudero, Miguel Angel
dc.date.available
2016-12-26T14:40:02Z
dc.date.issued
2013-03
dc.identifier.citation
Borgonovo, Janina Edith; Troncoso, Mariana Elizabeth; Lucas, José J.; Sosa Escudero, Miguel Angel; Mutant huntingtin affects endocytosis in striatal cells by altering the binding of AP-2 to membranes; Elsevier; Experimental Neurology; 241; 3-2013; 75-83
dc.identifier.issn
0014-4886
dc.identifier.uri
http://hdl.handle.net/11336/10077
dc.description.abstract
Clathrin-mediated endocytosis plays an important role in the maintenance of neuronal integrity in the synaptic terminals. Here we studied the effect of anomalous polyglutamine expansion in huntingtin on the interaction of coat proteins with membranes, in areas of mouse brain or in cultured striatal cells. We observed that this anomaly induces a redistribution of AP-2, but not other coat proteins, from the membrane to the cytosol in the striatum, and in the cultured striatal cells. It was also noted that huntingtin associates with AP-2, and that this association decreases due to the mutation in huntingtin. This decreased receptor-mediated endocytosis, measured by the internalization of transferrin in the mutated cells. It was also confirmed that huntingtin mutation made the cells more vulnerable to the action of quinolinic acid, with an increasing degradation of the AP-2 alpha subunits. On the basis of these results, we conclude that abnormal polyglutamine expansion in huntingtin affects clathrin-mediated endocytosis, and may be one of the pathogenic mechanisms of neurodegeneration.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Endocytosis
dc.subject
Clathrin Coated Vesicles
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Neurodegeneration
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Huntingtin
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Huntington'S Disease
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Neurociencias
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Mutant huntingtin affects endocytosis in striatal cells by altering the binding of AP-2 to membranes
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-09-19T18:45:55Z
dc.journal.volume
241
dc.journal.pagination
75-83
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Borgonovo, Janina Edith. Consejo Nacional de Investigaciones Cientficas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto Histología y Embriología de Mendoza "Dr. M. Burgos"; Argentina
dc.description.fil
Fil: Troncoso, Mariana Elizabeth. Consejo Nacional de Investigaciones Cientficas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto Histología y Embriología de Mendoza "Dr. M. Burgos"; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
dc.description.fil
Fil: Lucas, José J.. Universidad Autónoma de Madrid. Consejo superior de Investigaciones Científicas. Centro de Biología Molecular "Severo Ochoa"; España
dc.description.fil
Fil: Sosa Escudero, Miguel Angel. Consejo Nacional de Investigaciones Cientficas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto Histología y Embriología de Mendoza "Dr. M. Burgos"; Argentina
dc.journal.title
Experimental Neurology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0014488612004426
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.expneurol.2012.11.025
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