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dc.contributor.author
Ruckebusch, Cyril
dc.contributor.author
Bernex, Romain
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Allegrini, Franco
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Sliwa, Michel
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Hofkens, Johan
dc.contributor.author
Dedecker, Peter
dc.date.available
2020-03-25T18:30:45Z
dc.date.issued
2015-05
dc.identifier.citation
Ruckebusch, Cyril; Bernex, Romain; Allegrini, Franco; Sliwa, Michel; Hofkens, Johan; et al.; Mapping pixel dissimilarity in wide-field super-resolution fluorescence microscopy; American Chemical Society; Analytical Chemistry; 87; 9; 5-2015; 4675-4682
dc.identifier.issn
0003-2700
dc.identifier.uri
http://hdl.handle.net/11336/100747
dc.description.abstract
Recent advances in fluorescence bioimaging with single-molecule sensitivity have relied on the analysis and visualization of single-molecule data obtained on smart fluorophores. We describe an alternative method to enhance the information content of densely labeled fluorescence images. Visualization is improved by representing pixels as the dissimilarities of the fluctuations of the fluorescence signals, with the dissimilarity being taken to the mean of the signals over all the pixels. Mapping pixel dissimilarity (Mappix) results in signal and information enhancement of the output images. In addition, the spatial distribution of the fluorescence brightness of the original image is not skewed. This allows large differences of molecular brightness to be handled which turns out to be critical to the fidelity of the final image. In this work, we provide testing of the Mappix approach with both simulated and real data. The results obtained on HEK cells expressing Dronpa photoswitchable fluorescent protein show that, for densely labeled samples, improvement can be obtained on fluorescence images allowing the observation of structural information. Despite some limitations, comparison to state of art methods reveals that Mappix can be very useful for biological imaging applications.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Chemical Society
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Superresolution Fluorescence Microscopy
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Pixel dissimilarity
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Single molecule visualization
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Biological Imaging
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Química Analítica
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Mapping pixel dissimilarity in wide-field super-resolution fluorescence microscopy
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-03-25T13:27:41Z
dc.journal.volume
87
dc.journal.number
9
dc.journal.pagination
4675-4682
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Washington
dc.description.fil
Fil: Ruckebusch, Cyril. Universite de Lille I; Francia
dc.description.fil
Fil: Bernex, Romain. Universite de Lille I; Francia
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Fil: Allegrini, Franco. Universite de Lille I; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; Argentina
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Fil: Sliwa, Michel. Universite de Lille I; Francia
dc.description.fil
Fil: Hofkens, Johan. Katholikie Universiteit Leuven; Bélgica
dc.description.fil
Fil: Dedecker, Peter. Katholikie Universiteit Leuven; Bélgica
dc.journal.title
Analytical Chemistry
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1021/ac504295p
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/ac504295p
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